Paper
7 October 2014 Complex of the new generation of the instrumental analytical approaches to prevent dangerous bioterrorism
Author Affiliations +
Abstract
The real manifestations of biowarfare were only during local military conflicts. In previous times the danger with this factor forms through activation of terrorist organizations. To prevent non-desirable consequences and to preserve the lives and health of people it is necessary to provide constant control by the application of complex of the new generation of the instrumental devices based on the principles of biosensorics which allow rapid revealing step by step: total toxicity of environmental objects, presence of appropriate groups and specific chemical substances among them. It is demonstrated simple and rapid estimation of the total toxicity through the control of the intensity of chlorophyll fluorescence (IChF) by the direct or remote ways by the device “Floratest” (Ukraine). There is possible on the basis of IChF curve of growing plants from some territory during appropriate period to reveal appearance of toxic substances in this area. Their presence in more local cases may be done by using bioluminescent bacteria (pure Ukrainian strains) or/and controlling short term Daphnia living medium by developed portable chemiluminometer. For the revealing of some groups of toxic elements it is recommended the cerium oxide ISFETs based enzymatic biosensors. The last and devices based on SPR (“Plasmotest”, Ukraine), porous silicon (with the registration of biospecific interaction macromolecules by luminescence or electro conductivity) and some nano-metal oxides were realized in immune biosensors at the determination of content of number of mycotoxins, some microorganisms (Salmonella spp.) and diagnostics of viral disease (retroviral leucosis). We present the main characteristics of the above mentioned devices and give confirmation that all the analysis meets practice demands. Overall time of analysis is in range 10 min and it is very simple and may be realized in field conditions.
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Nickolaj F. Starodub, Kateryna Shavanova, and Andrii Karpiuk "Complex of the new generation of the instrumental analytical approaches to prevent dangerous bioterrorism", Proc. SPIE 9253, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence X; and Optical Materials and Biomaterials in Security and Defence Systems Technology XI, 92530B (7 October 2014); https://doi.org/10.1117/12.2066070
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KEYWORDS
Biosensors

Biological research

Luminescence

Bacteria

Field effect transistors

Toxicity

Diagnostics

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