Experimental demonstration and theoretical explanation of the efficiency of the nano-structured silicon as the transducer for optical immune biosensors

Nickolaj F. Starodub; Nelya F. Slyshyk; Kateryna E. Shavanova; Andrij Karpyuk; Mykola M. Mel’nichenko; Anatolij V. Zherdev; Boris B. Dzantiev

doi:10.1117/12.2081163

22 October 2014 Experimental demonstration and theoretical explanation of the efficiency of the nano-structured silicon as the transducer for optical immune biosensors

Nickolaj F. Starodub, Nelya F. Slyshyk, Kateryna E. Shavanova, Andrij Karpyuk, Mykola M. Mel’nichenko, Anatolij V. Zherdev, Boris B. Dzantiev

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Proceedings Volume 9421, Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8); 94210F (2014) https://doi.org/10.1117/12.2081163
Event: Eighth International Conference on Advanced Optical Materials and Devices, 2014, Riga, Latvia

Abstract

It is presented the experimental results about the investigations of the efficiency of the structured nano-pourous silicon (sNPS) application as transducer in the immune biosensors designed for the control of retroviral bovine leucosis (RBL) and the determination of the level such mycotoxins as T2 and patulin among environmental objects. Today, there is an arsenal of the traditional immunological methods that allow for the biochemical diagnostics of the above diseases and control of toxins but they are deeply routine and can not provide the requirements of practice for express analysis, its low cost and simplicity. Early to provide practical demands we developed immune biosensors based on SPR, TIRE and thermistors. To find more simple variant of the assay we studied the efficiency sNPS as trasducer in immune biosensor. The registration of the specific signals was made by measuremets of level of chemiluminescence (ChL) or photocurrent. The sensitivity of biosensor for both variants of the specific signal registration at the determination of T2 and patulin was about 10-20 ng/ml. Sensitivity analysis of RBL by this immune biosensors exceeds traditionally used approaches including the ELISA-method too. The optimal serum dilution of blood at the screening leukemia should be no less than 1:100, or even 1:500. The immune biosensor may be applied too for express screening leucosis through analysis of milk. In this case the optimal serum dilution of milk should be about 1:20. The total time of analysis including all steps (immobilization of specific Ab or antigens on the transducer surface and measurements) was about 40 min and it may be a sharp decline if the above mentione sensitive elements will be immobilized preliminary measurements. It is concluded that the proposed type of transducer for immune biosensor is effective for analysis of mycotoxins in screening regime.

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Nickolaj F. Starodub, Nelya F. Slyshyk, Kateryna E. Shavanova, Andrij Karpyuk, Mykola M. Mel’nichenko, Anatolij V. Zherdev, and Boris B. Dzantiev "Experimental demonstration and theoretical explanation of the efficiency of the nano-structured silicon as the transducer for optical immune biosensors", Proc. SPIE 9421, Eighth International Conference on Advanced Optical Materials and Devices (AOMD-8), 94210F (22 October 2014); https://doi.org/10.1117/12.2081163

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KEYWORDS

Biosensors

Biological research

Silicon

Transducers

Diagnostics

Blood

Etching

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