Cell trapping in a blood capillary phantom using laser tweezers

Sergei S. Klykov; Ivan V. Fedosov; Valery V. Tuchin

doi:10.1117/12.2179976

19 March 2015 Cell trapping in a blood capillary phantom using laser tweezers

Sergei S. Klykov, Ivan V. Fedosov, Valery V. Tuchin

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480A (2015) https://doi.org/10.1117/12.2179976
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

As a phantom of native blood capillary the plastic capillary tube and as a model of red blood cells the yeast cells are considered. Plastic capillary has circular a cross-section with diameter ranging between 20 and 40 μ. For velocity estimation of polystyrene beads which had a role of tracers in water the particle image velocimetry method is realized using NI Labview Vision standard functions of image processing. It is shown that in spite of the presence of uncompensated spherical aberration emerging from refraction incident beam in curved plastic capillary walls yeast cells can be confined in stable 3D trap.

Citation Download Citation

Sergei S. Klykov, Ivan V. Fedosov, and Valery V. Tuchin "Cell trapping in a blood capillary phantom using laser tweezers", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480A (19 March 2015); https://doi.org/10.1117/12.2179976

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