Extending the depth-of-field for microscopic imaging by means of multifocus color image fusion

R. Hurtado-Pérez; C. Toxqui-Quitl; A. Padilla-Vivanco; G. Ortega-Mendoza

doi:10.1117/12.2188927

3 September 2015 Extending the depth-of-field for microscopic imaging by means of multifocus color image fusion

R. Hurtado-Pérez, C. Toxqui-Quitl, A. Padilla-Vivanco, G. Ortega-Mendoza

Proceedings Volume 9578, Current Developments in Lens Design and Optical Engineering XVI; 957811 (2015) https://doi.org/10.1117/12.2188927
Event: SPIE Optical Engineering + Applications, 2015, San Diego, California, United States

Abstract

In microscopy, the depth of field (DOF) is limited by the physical characteristics of imaging systems. Imaging a scene with the all the field of view in focus can be an impossible task to achieve. In this paper, metal samples are inspected on multiple focal planes by moving the microscope stage along the z − axis and for each z plane, an image is digitalized. Through digital image processing, an image with all the focused regions is generated from a set of multi focus images. The proposed fusion algorithm gives a single sharp image. The merger scheme is simple, fast and virtually free of artifacts or false color. Experimental fusion results are shown.

Citation Download Citation

R. Hurtado-Pérez, C. Toxqui-Quitl, A. Padilla-Vivanco, and G. Ortega-Mendoza "Extending the depth-of-field for microscopic imaging by means of multifocus color image fusion", Proc. SPIE 9578, Current Developments in Lens Design and Optical Engineering XVI, 957811 (3 September 2015); https://doi.org/10.1117/12.2188927

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