Concurrent fluorescence macro-imaging across multiple spectral regions in the visible and the near infrared

Farnoud Kazemzadeh; Shahid Haider; Chao Jin; David A. Clausi; Alexander Wong

doi:10.1117/12.2188209

3 September 2015 Concurrent fluorescence macro-imaging across multiple spectral regions in the visible and the near infrared

Farnoud Kazemzadeh, Shahid Haider, Chao Jin, David A. Clausi, Alexander Wong

Author Affiliations +

Proceedings Volume 9579, Novel Optical Systems Design and Optimization XVIII; 957907 (2015) https://doi.org/10.1117/12.2188209
Event: SPIE Optical Engineering + Applications, 2015, San Diego, California, United States

Abstract

Fluorescent imaging, often synonymous with microscopic imaging, is an imaging modality whereby various features of a target are observed based on assignment of chemical labels. These labels are in most cases indirect tracers of specific structures or chemical compounds which cannot be otherwise identified. The tracers are excited by an illuminating source and they in turn emit light at specific wavelengths. This light is then captured by an imaging device and represented as an indirect observation of the specific feature in the sample. The process of excitation and imaging of the emitted light is performed sequentially and is proportional to the number of tracers or fluorescence species present in the sample. We present an imaging system that can image fluorescent tracers, in the visible and the near Infra-red, simultaneously. This system is capable of illuminating the target with different excitation light sources and capture the corresponding fluorescence images in one snapshot using a series of mirrors to capture different views of the sample. The simultaneously captured image are fused using a computational reconstruction process to present a coherent multispectral fluorescence image. The system is proposed for use in applications where the rapid enumeration of fluorescent species in a large field of view is paramount as opposed to their microscopic image in a narrow field of view. The system was tested using a controlled cocktail solution of four different types fluorescent microspheres and was able to enumerate the microspheres based on their different fluorescent signatures as captured by the system.

Citation Download Citation

Farnoud Kazemzadeh, Shahid Haider, Chao Jin, David A. Clausi, and Alexander Wong "Concurrent fluorescence macro-imaging across multiple spectral regions in the visible and the near infrared", Proc. SPIE 9579, Novel Optical Systems Design and Optimization XVIII, 957907 (3 September 2015); https://doi.org/10.1117/12.2188209

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Imaging systems

Luminescence

Cameras

Sensors

Visible radiation

Near infrared

Optical filters

Show All Keywords

Keywords/Phrases

Search In:

Publication Years