A practical optical-resolution photoacoustic microscopy prototype using a 300 mW visible laser diode

Lvming Zeng; Zhonglie Piao; Shenghai Huang; Wangcun Jia; Zhongping Chen

doi:10.1117/12.2209201

15 March 2016 A practical optical-resolution photoacoustic microscopy prototype using a 300 mW visible laser diode

Lvming Zeng, Zhonglie Piao, Shenghai Huang, Wangcun Jia, Zhongping Chen

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97084T (2016) https://doi.org/10.1117/12.2209201
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique for microvasculature imaging at high spatial resolution and contrast. In this work, we present a practical visible laser-diode-based OR-PAM (LD-OR-PAM) prototype for vasculature imaging, which has the desirable properties of being portable, low-cost, and label-free. The prototype employs a 300 mW pulsed laser diode in a 3.8 mm diameter package, emitting 174 ns pulses at 405 ± 5 nm wavelength and a pulse energy of 52 nJ. An aspheric objective with a numerical aperture of 0.60 is used to achieve microscope optical illumination. The laser diode excitation has a compact size of 4.5 × 1.8 × 1.8 cm³ assembled with a cooling block. The lateral resolution was tested to be 0.95 μm on ~7 μm carbon fibers. The subcutaneous microvasculature on a mouse back was label-free imaged ex vivo, which demonstrates the potential of the LD-OR-PAM prototype for in vivo imaging skin chromosphores such as hemoglobin. Our ultimate aim is to provide a practical and affordable OR-PAM system as a routine instrument for standard clinical applications.

Citation Download Citation

Lvming Zeng, Zhonglie Piao, Shenghai Huang, Wangcun Jia, and Zhongping Chen "A practical optical-resolution photoacoustic microscopy prototype using a 300 mW visible laser diode", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97084T (15 March 2016); https://doi.org/10.1117/12.2209201

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KEYWORDS

Semiconductor lasers

Photoacoustic microscopy

Optical fibers

Biomedical optics

Imaging systems

Objectives

Signal detection

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