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28 April 2022 Multi-plane 3D optical voltage imaging using high-speed multi-Z confocal microscopy
Timothy D. Weber, Maria V. Moya, Michael N. Economo, Jerome Mertz
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Proceedings Volume PC11946, Neural Imaging and Sensing 2022; PC1194606 (2022) https://doi.org/10.1117/12.2607867
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Fluorescent genetically encoded voltage indicators can be combined with optical imaging to provide high-throughput electrophysiologic recordings with single-spike resolution and subthreshold sensitivity. Such voltage imaging is highly demanding in terms of signal collection; thus, most experiments have been performed with widefield one-photon microscopy. Unfortunately, widefield techniques are susceptible to out-of-focus background and scattering, which degrades SNR, especially in high-density slice or in vivo experiments. In this work, we describe a multi-plane near-kHz-rate confocal microscope that effectively suppresses undesired background. This technique enables more densely labeled in vitro and in vivo imaging experiments, critical for the dissection of neural circuit dynamics.
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Timothy D. Weber, Maria V. Moya, Michael N. Economo, and Jerome Mertz "Multi-plane 3D optical voltage imaging using high-speed multi-Z confocal microscopy", Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC1194606 (28 April 2022); https://doi.org/10.1117/12.2607867
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KEYWORDS
Confocal microscopy
Optical imaging
3D image processing
Stereoscopy
In vivo imaging
In vitro testing
Microscopes
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