Endogenous transmission optical tomography of biological samples: static and dynamic approaches

Albert Claude Boccara; Olivier Thouvenin; Linh Giang Pham; Martine Boccara

doi:10.1117/12.2607849

2 March 2022 Endogenous transmission optical tomography of biological samples: static and dynamic approaches

Albert Claude Boccara, Olivier Thouvenin, Linh Giang Pham, Martine Boccara

Author Affiliations +

Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720L (2022) https://doi.org/10.1117/12.2607849
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Full Field Optical Transmission Tomography (FFOTT) relies on forward scattering using the Gouy’s phase shift modulation that is achieved close to the focus of a microscope objective. FFOTT principle is different than Full Field OCT (FFOCT) that relies on backscattering of light, This new type of endogenous cell imaging technique offers structural and metabolic contrasts and is particularly well suited for imaging cell culture on glass slide or Petri dishes avoiding fringes that mask cells in FFOCT as well as biological structures such as biofilms. The sectioning ability is close to confocal microscopy but no contrast agent is required.

Conference Presentation

Citation Download Citation

Albert Claude Boccara, Olivier Thouvenin, Linh Giang Pham, and Martine Boccara "Endogenous transmission optical tomography of biological samples: static and dynamic approaches", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720L (2 March 2022); https://doi.org/10.1117/12.2607849

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