Presentation
2 March 2022 Volumetric differential-phase imaging in scattering mode by optical coherence tomography
Author Affiliations +
Abstract
We demonstrate volumetric phase contrast imaging by using optical coherence tomography (OCT). In general, the randomness of the scatterers’ distribution prohibits the volumetric measurement of a meaningful phase in a scattering mode. Our method uses complex numerical manipulation of an en-face complex OCT and gives a transversally differential phase image similar to a differential interference contrast microscope (DIC). Not like the DIC, our method can arbitrarily select the amount and direction of the shear after the OCT acquisition. In addition it provides DIC-like images at arbitrary depths. This method is validated by using a 840-nm spectral domain OCT system. A zebrafish sample is measured over a 1-mm × 1-mm transversal scanning range.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kiriko Tomita, Daisuke Oida, Antonia Lichtenegger, Makoto Kobayashi, Junya Tamaoki, Lixuan Bian, Shuichi Makita, Kensuke Oikawa, Rion Morishita, and Yoshiaki Yasuno "Volumetric differential-phase imaging in scattering mode by optical coherence tomography", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720O (2 March 2022); https://doi.org/10.1117/12.2608606
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KEYWORDS
Optical coherence tomography

Light scattering

Scattering

Phase measurement

Refractive index

Digital image correlation

Tissue optics

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