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16 March 2023 Automated hologram reconstruction, including fast defocus correction, for developing time-lapse polychromatic digital holographic microscopy (Conference Presentation)
Mohamed Haouat, Céline Larivière-Loiselle, Maxime Moreaud, Erik Bélanger, Pierre Marquet
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Proceedings Volume PC12389, Quantitative Phase Imaging IX; PC123890D (2023) https://doi.org/10.1117/12.2647909
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Polychromatic digital holographic microscopy (P-DHM) has proven its capacity to provide quasi-coherent noise-free quantitative-phase images, allowing a high-quality visualization of cell structure. In this work we propose a fully automated hologram reconstruction methodology, including a fast-numerical approach for the correction of the defocusing resulting from both the axial chromatic aberrations as well as the fluctuations of the optomechanical elements. This methodology able to reconstruct a large number of holograms paves the way to develop a time-resolved P-DHM capable of non-invasively visualizing both the fine cell structure and dynamics.
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Mohamed Haouat, Céline Larivière-Loiselle, Maxime Moreaud, Erik Bélanger, and Pierre Marquet "Automated hologram reconstruction, including fast defocus correction, for developing time-lapse polychromatic digital holographic microscopy (Conference Presentation)", Proc. SPIE PC12389, Quantitative Phase Imaging IX, PC123890D (16 March 2023); https://doi.org/10.1117/12.2647909
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KEYWORDS
Digital holography
Holograms
Holography
Microscopy
Time lapse microscopy
3D image reconstruction
Chromatic aberrations
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