16 March 2023High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)
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Nonlinear label-free microscopy techniques have shown great promise for analyzing biological tissues with an unmatched level of information. These techniques are capable of identifying intrinsic optical signals generated in response to ultrafast laser pulses. A roadblock in fast label-free imaging is the limited signal-to-noise ratio (SNR), due to the low number of photons close to the noise level. We implemented heterodyne detection of the third-order signals to overcome this limit and surpass the 1/f noise. Our method increases the SNR by a factor of 2.3, leading to a factor of 5 reduction in imaging time.
Kayvan F. Tehrani,Alejandro De la Cadena, andStephen A. Boppart
"High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239009 (16 March 2023); https://doi.org/10.1117/12.2648069
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Kayvan F. Tehrani, Alejandro De la Cadena, Stephen A. Boppart, "High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)," Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239009 (16 March 2023); https://doi.org/10.1117/12.2648069