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15 March 2023 Video-rate mid-infrared photothermal imaging of cellular dynamics (Conference Presentation)
Jiaze Yin, Lu Lan, Meng Zhang, Fukai Chen, Zhongyue Guo, Ji-Xin Cheng
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239204 (2023) https://doi.org/10.1117/12.2650764
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The imaging speed of the current mid-infrared photothermal (MIP) microscope is limited to tens of seconds per frame due to the long pixel dwell time and slow sample scanning process, which is insufficient for capturing dynamics inside living systems. In this work, we developed a video-rate MIP microscope by employing a lock-in free demodulation scheme to resolve single IR pulse induced contrast. We further developed a synchronous pump-probe Galvo scanning for reaching a line rate over 2.5 kHz. The system is capable of resolving chemical dynamics in living cells in a uniform imaging field of view over 300 μm.
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Jiaze Yin, Lu Lan, Meng Zhang, Fukai Chen, Zhongyue Guo, and Ji-Xin Cheng "Video-rate mid-infrared photothermal imaging of cellular dynamics (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239204 (15 March 2023); https://doi.org/10.1117/12.2650764
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KEYWORDS
Imaging systems
Mid-IR
Infrared imaging
Microscopes
Mirrors
Optical sensing
Photothermal effect
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