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Application of compressed imaging methods in optics has led to advancements in high-speed snapshot imaging such as Light Field Tomography (LIFT). LIFT utilizes computed tomography and light field imaging to compress a snapshot 3-D field of view into a 1-D line of light. It can achieve excellent imaging speeds when used with a high-speed linear sensor. We propose using LIFT to image multi-neuron dynamics with the ASAP voltage indicator, which has proven challenging to image due to millisecond dynamics and low brightness. Here we report a prototype system to confirm performance by imaging high speed GCaMP Calcium Indicator dynamics.
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Ohr Benshlomo, Conor Dorian, Jorge Tordera Mora, Aryan Palmar, Peyman Golshani, Liang Gao, "Snapshot 3D imaging with light field tomography," Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128571F (13 March 2024); https://doi.org/10.1117/12.3001980