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13 March 2024 Real-time wide-field broadband CARS microscopy
Chiara Ceconello, Federico Vernuccio, Andrea Rabolini, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, Dario Polli
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Proceedings Volume PC12870, Real-time Measurements, Rogue Phenomena, and Single-Shot Applications IX; PC1287005 (2024) https://doi.org/10.1117/12.3002962
Event: SPIE LASE, 2024, San Francisco, California, United States
Abstract
Coherent anti-Stokes Raman scattering (CARS) is a highly effective Non-Linear Optical (NLO) microscopy technique for label-free vibrational imaging of unperturbed samples. We present a novel scheme for video-rate wide-field CARS microscopy over a wide (up to 100-μm diameter) field of view, based on an Ytterbium amplified laser followed by white-light generation and optical parametric amplification. It records the full fingerprint region of the molecular vibrational spectrum and enables chemically-specific real-time characterization of fast dynamics with down to few-milliseconds time resolution. The system can perform either real-time imaging at single Raman shifts or acquire three-dimensional hypercubes, chemically richer in information.
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Chiara Ceconello, Federico Vernuccio, Andrea Rabolini, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, and Dario Polli "Real-time wide-field broadband CARS microscopy", Proc. SPIE PC12870, Real-time Measurements, Rogue Phenomena, and Single-Shot Applications IX, PC1287005 (13 March 2024); https://doi.org/10.1117/12.3002962
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KEYWORDS
Microscopy
Vibration
Nonlinear optics
Pulse signals
Raman spectroscopy
Signal generators
Supercontinuum generation
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