Multifunctional darkfield microscopy using an axicon

Ming Lei; Baoli Yao

doi:10.1117/1.2960019

1 July 2008 Multifunctional darkfield microscopy using an axicon

Ming Lei, Baoli Yao

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Journal of Biomedical Optics, Vol. 13, Issue 4, 044024 (July 2008). https://doi.org/10.1117/1.2960019

Abstract

We present a multifunctional darkfield microscopy using an axicon. It combines the functions of a darkfield microscope, fluorescence microscope, and microspectrophotometer in one platform. The advantage of the system over conventional darkfield microscopy includes the high transmittance of the illuminating flux, the high contrast of the image, and the convenient toggle between darkfield and brightfield microscopy. Examples of dark, bright, and fluorescent micrographs as well as concerned spectra of microsized specimens implemented in this apparatus are demonstrated.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Ming Lei and Baoli Yao "Multifunctional darkfield microscopy using an axicon," Journal of Biomedical Optics 13(4), 044024 (1 July 2008). https://doi.org/10.1117/1.2960019

Published: 1 July 2008

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