Antibody-labeled fluorescence imaging of dendritic cell populations in vivo

Ryan J. Cummings; Soumya Mitra; Edith M. Lord; Thomas H. Foster

doi:10.1117/1.2966122

1 July 2008 Antibody-labeled fluorescence imaging of dendritic cell populations in vivo

Ryan J. Cummings, Soumya Mitra, Edith M. Lord, Thomas H. Foster

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Journal of Biomedical Optics, Vol. 13, Issue 4, 044041 (July 2008). https://doi.org/10.1117/1.2966122

Abstract

We report an optical molecular imaging technique that exploits local administration of fluorophore-conjugated antibodies and confocal fluorescence microscopy to achieve high-contrast imaging of host cell populations in normal and tumor tissue in living mice. The method achieves micron-scale spatial resolution to depths greater than 100 μm. We illustrate the capabilities of this approach by imaging two dendritic cell populations in the skin and normal and tumor vasculature in vivo.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Ryan J. Cummings, Soumya Mitra, Edith M. Lord, and Thomas H. Foster "Antibody-labeled fluorescence imaging of dendritic cell populations in vivo," Journal of Biomedical Optics 13(4), 044041 (1 July 2008). https://doi.org/10.1117/1.2966122

Published: 1 July 2008

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