Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy

Deqiu Huang; Wen Zhang; Huiqing Zhong; Honglian Xiong; Xi Guo; Zhouyi Guo

doi:10.1117/1.JBO.17.1.015004

6 February 2012 Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy

Deqiu Huang, Wen Zhang, Huiqing Zhong, Honglian Xiong, Xi Guo, Zhouyi Guo

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Journal of Biomedical Optics, Vol. 17, Issue 1, 015004 (February 2012). https://doi.org/10.1117/1.JBO.17.1.015004

Abstract

In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved.

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Deqiu Huang, Wen Zhang, Huiqing Zhong, Honglian Xiong, Xi Guo, and Zhouyi Guo "Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy," Journal of Biomedical Optics 17(1), 015004 (6 February 2012). https://doi.org/10.1117/1.JBO.17.1.015004

Published: 6 February 2012

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