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6 November 2013 Microfluidic approach for direct and uniform laser irradiation to study biochemical state changes on Jurkat-T cells
Sween J. Butler, Dong-Weon Lee, Curtis W. Burney, Jeffrey C. Wigle, Tae-Youl Choi
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Journal of Biomedical Optics, Vol. 18, Issue 11, 117004 (November 2013). https://doi.org/10.1117/1.JBO.18.11.117004
Abstract
We investigated the potential for using polydimethylsiloxane microfluidic devices in a biological assay to explore the cellular stress response (CSR) associated with hyperthermia induced by exposure to laser radiation. In vitro studies of laser-tissue interaction traditionally involved exposing a monolayer of cells. Given the heating-cooling dynamics of the cells and nutrient medium, this technique produces a characteristic “bulls-eye” temperature history that plagues downstream molecular analyses due to the nonuniform thermal experience of exposed cells. To circumvent this issue, we devised an approach to deliver single cells to the laser beam using a microfluidic channel, allowing homogeneous irradiation and collection of sufficient like-treated cells to measure changes in CSR after laser heating. To test this approach, we irradiated Jurkat-T cells with a 2-μm-wavelength laser in one branch of a 100-μm-wide bifurcated channel while unexposed control cells were simultaneously passing through the other, identical channel. Cell viability was measured using vital dyes, and expression of HSPA1A was measured using reverse transcription polymerase chain reaction. The laser damage threshold was 25±2  J/cm 2 , and we found a twofold increase in expression at that exposure. This approach may be employed to examine transcriptome-wide/proteome changes and further comparative work across stressors and cell types.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Sween J. Butler, Dong-Weon Lee, Curtis W. Burney, Jeffrey C. Wigle, and Tae-Youl Choi "Microfluidic approach for direct and uniform laser irradiation to study biochemical state changes on Jurkat-T cells," Journal of Biomedical Optics 18(11), 117004 (6 November 2013). https://doi.org/10.1117/1.JBO.18.11.117004
Published: 6 November 2013
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Cited by 2 scholarly publications.
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KEYWORDS
Microfluidics
Laser irradiation
Laser tissue interaction
Biological research
Laser damage threshold
Photoresist materials
Proteins
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