Multifrequency synthesis and extraction using square wave projection patterns for quantitative tissue imaging

Kyle P. Nadeau; Tyler B. Rice; Anthony J. Durkin; Bruce J. Tromberg

doi:10.1117/1.JBO.20.11.116005

2 November 2015 Multifrequency synthesis and extraction using square wave projection patterns for quantitative tissue imaging

Kyle P. Nadeau, Tyler B. Rice, Anthony J. Durkin, Bruce J. Tromberg

Author Affiliations +

Funded by: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), National Institutes of Health, NIH, National Institute of Health, NIH/NIBIB, Beckman Foundation and the NIH, NIH (NIBIB) Laser Microbeam and Medical Program, NIBIB Biomedical Technology Research Center LAMMP, NIH NIBIB Biomedical Technology Research Center LAMMP, NIH/NIBIB funded LAMMP, US National Institutes of Health (NIH), National Institute of Biomedical Imaging and Bioengineering, Arnold & Mabel Beckman Foundation, National Institute of Biomedical Imaging Bioengineering, NIH NIBIB, NIH (NINDS), Military Medical Photonics Program, AFOSR, Air Force Office of Scientific Research (AFOSR), AFOSR, Military Photomedicine Program, Military Photomedicine Program, AFOSR, National Institute of Environmental Health Sciences (NIEHS), NSF IGERT, Air Force Office of Scientific Research Medical Free-Electron Laser Program, Beckman Foundation

Journal of Biomedical Optics, Vol. 20, Issue 11, 116005 (November 2015). https://doi.org/10.1117/1.JBO.20.11.116005

Abstract

We present a method for spatial frequency domain data acquisition utilizing a multifrequency synthesis and extraction (MSE) method and binary square wave projection patterns. By illuminating a sample with square wave patterns, multiple spatial frequency components are simultaneously attenuated and can be extracted to determine optical property and depth information. Additionally, binary patterns are projected faster than sinusoids typically used in spatial frequency domain imaging (SFDI), allowing for short (millisecond or less) camera exposure times, and data acquisition speeds an order of magnitude or more greater than conventional SFDI. In cases where sensitivity to superficial layers or scattering is important, the fundamental component from higher frequency square wave patterns can be used. When probing deeper layers, the fundamental and harmonic components from lower frequency square wave patterns can be used. We compared optical property and depth penetration results extracted using square waves to those obtained using sinusoidal patterns on an in vivo human forearm and absorbing tube phantom, respectively. Absorption and reduced scattering coefficient values agree with conventional SFDI to within 1% using both high frequency (fundamental) and low frequency (fundamental and harmonic) spatial frequencies. Depth penetration reflectance values also agree to within 1% of conventional SFDI.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Kyle P. Nadeau, Tyler B. Rice, Anthony J. Durkin, and Bruce J. Tromberg "Multifrequency synthesis and extraction using square wave projection patterns for quantitative tissue imaging," Journal of Biomedical Optics 20(11), 116005 (2 November 2015). https://doi.org/10.1117/1.JBO.20.11.116005

Published: 2 November 2015

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