High-performance, inexpensive setup for simultaneous multisite recording of electrophysiological signals and mesoscale voltage imaging in the mouse cortex

Edgar Bermudez-Contreras; Sergey Chekhov; Jianjun Sun M.D.; Jennifer Tarnowsky; Bruce L. McNaughton; Majid H. Mohajerani

doi:10.1117/1.NPh.5.2.025005

29 March 2018 High-performance, inexpensive setup for simultaneous multisite recording of electrophysiological signals and mesoscale voltage imaging in the mouse cortex

Edgar Bermudez-Contreras, Sergey Chekhov, Jianjun Sun M.D., Jennifer Tarnowsky, Bruce L. McNaughton, Majid H. Mohajerani

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Neurophotonics, Vol. 5, Issue 2, 025005 (March 2018). https://doi.org/10.1117/1.NPh.5.2.025005

Abstract

Simultaneous recording of optical and electrophysiological signals from multiple cortical areas may provide crucial information to expand our understanding of cortical function. However, the insertion of multiple electrodes into the brain may compromise optical imaging by both restricting the field of view and interfering with the approaches used to stabilize the specimen. Existing methods that combine electrophysiological recording and optical imaging in vivo implement either multiple surface electrodes, silicon probes, or a single electrode for deeper recordings. To address such limitation, we built a microelectrode array (hyperdrive, patent US5928143 A) compatible with wide-field imaging that allows insertion of up to 12 probes into a large brain area (8 mm diameter). The hyperdrive is comprised of a circle of individual microdrives where probes are positioned at an angle leaving a large brain area unobstructed for wide-field imaging. Multiple tetrodes and voltage-sensitive dye imaging were used for acute simultaneous registration of spontaneous and evoked cortical activity in anesthetized mice. The electrophysiological signals were used to extract local field potential (LFP) traces, multiunit, and single-unit spiking activity. To demonstrate our approach, we compared LFP and VSD signals over multiple regions of the cortex and analyzed the relationship between single-unit and global cortical population activities. The study of the interactions between cortical activity at local and global scales, such as the one presented in this work, can help to expand our knowledge of brain function.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Edgar Bermudez-Contreras, Sergey Chekhov, Jianjun Sun M.D., Jennifer Tarnowsky, Bruce L. McNaughton, and Majid H. Mohajerani "High-performance, inexpensive setup for simultaneous multisite recording of electrophysiological signals and mesoscale voltage imaging in the mouse cortex," Neurophotonics 5(2), 025005 (29 March 2018). https://doi.org/10.1117/1.NPh.5.2.025005

Received: 21 November 2017; Accepted: 5 March 2018; Published: 29 March 2018

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