Two-photon vision relies on the perception of pulsed near-infrared laser beams as having colors like their half-wavelength counterparts. The phenomenon is due to two-photon absorption occurring in visual pigments [1]. This study is focused on methods to determine the contrast sensitivity function (CSF) for two-photon vision, which has not yet been investigated. CSF was measured for eight spatial frequencies using the tumbling E letter optotype. The optotype was projected on a white background by fast scanning the retina with a pulsed 1040 nm or 520 nm laser beam, both perceived as green. The contrast threshold was determined for the power of the beam corresponding to a minimum stimulus brightness for which the subject was able to state the correct letter orientation. Because a luminance curve for the two-photon stimulus is not available, expressing the brightness of the infrared stimulus in photometric units required finding a suitable method. Three approaches for determining contrast sensitivity for two-photon stimulus were proposed and tested to overcome this problem. The threshold contrast values, defined as Weber contrast, differ substantially between normal and two-photon vision mechanisms. Each tested method allowed qualitative comparison of the obtained contrast sensitivities. The results show that the two-photon CSF has a significantly broader range than the one-photon CSF. Determining the CSF for twophoton vision will help assess the applicability of this phenomenon to augmented reality displays.
In this paper, we present the preliminary results of the scotopic luminosity curve for two-photon vision measurements in the spectral range from 872 nm to 1027 nm. The results were obtained thanks to a newly-developed custom-build tunable femtosecond erbium-doped fiber laser that pulse train parameters and spectral width are close to constant while tuning. Such instrumentation enabled us to perform reliable measurements across the laser tuning range of over 150 nm.
Light sensation relies on photoisomerization of chromophores in rod and cone photoreceptor cells. Spectral sensitivity of these photoreceptor cells in the retina is determined by the absorption spectra of their pigments which covers a range from 400 nm to above 700 nm. Regardless the mechanism leading to visual pigment isomerization, light sensation is triggered every time visual pigment molecules change their conformation. Thus, two-photon absorption (TPA) should produce the same result (visual sensation) as single photon absorption of light. This observation was positively verified and published by our group. During human psychophysics experiments, we found that humans can perceive light in the infrared (IR) range as colors that match half of the wavelength of the applied laser beam. Other experiments and theoretical research, such as mouse electrophysiology, biochemical studies of TPA in rhodopsin or molecular modeling studies, confirmed that visual sensation can be triggered by TPA. There are few publications describing human near infrared (NIR) perception and no formal proposals to use this phenomenon to improve ophthalmic diagnosis and monitor treatment. Here we report that the use of novel instrumentation revealed that the sensitivity threshold for NIR vision depends on age.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.