The problem of numerical simulations of the optical trap is widely discussed in literature for three regimes: geometrical optics for objects larger than wavelength of trapping laser, Lorenz-Mie regime for object’s size close to the wavelength and dipole approximation for trapped bead smaller than the wavelength [1]. Despite the abundance of articles in this field, the issue of comparison of numerical results with the experiment is omitted [2]. Here we show the compliance of numerical simulations with the experiment. By utilizing geometrical optics methods it is possible to numerically recreate behavior of beads for specific experimental setup. The parameters available in the simulation have their direct counterpart in the experimental system, which allows for comparison and verification of numerical simulations against the experiment. The data obtained from simulation and experiment is compared using statistical analysis of trajectory. Compliance of numerical simulations with experimental data proves useful for verification of methods of recording the object's trajectory in a trap.
We report the comprehensive application of double wavelength multifunctional optical tweezers. In the presented setup the measurement system is based on an inverted biological microscope. The system is equipped with two lasers of different wavelengths. The lasers’ beams, which generated optical traps, are controlled by galvanometer mirrors systems. Application of this setup has been presented in three examples. First one describes measurement of liposomes’ deformation, which is widely utilized in medical research. Secondly, the application of double wavelength multifunctional optical tweezers in precise control of micro-tools such as micro-dumbbells is presented. The third application of this system is the measurement of cell-to-cell adhesion forces, what is essential to understand the physical characteristics of living cells. In this paper we described preparation and process of samples measurement.
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