Ms. Arti Devi Profile

Arti Devi

at Saitama University

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Proceedings Article | 20 June 2024 Presentation + Paper

Fast and reliable micro bioassay techniques based on biospeckle for swift water assessment using plankton

Arti Devi, Hirofumi Kadono, Uma Maheswari Rajagopalan

Proceedings Volume 13008, 1300804 (2024) https://doi.org/10.1117/12.3022436

KEYWORDS: Microorganisms, Iron, Zinc, Toxicity, Biospeckle, Control systems, Metals, Tolerancing, Organisms, Imaging systems

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Recently, bioassay has been getting much attention as it can comprehensively evaluate water toxicity without identifying the individual chemical component. In this technique, a microscope observation is required to know the critical features of the targeted microorganisms. However, as the size of the microorganism becomes smaller, observation becomes more difficult due to the narrower focal depth of the imaging system. Here, to overcome these difficulties, a novel biospeckle technique is used for the microbioassay utilizing the biospeckle in the diffraction field generated from the microorganisms. Paramecium caudatum (200-300µm) and Euglena gracilis(50-60µm) were used as a microorganism. Dynamic biospeckle patterns were captured using a CCD camera to evaluate the swimming activity of microorganisms under varying water toxicity levels induced by heavy metal pollutants (Zn(NO₃)₂·6H₂O and FeSO₄·7H₂O). To quantitatively evaluate the swimming activity of microorganisms, the crosscorrelation function between the initial frame as a reference frame and the subsequent frames was measured, and the correlation time was evaluated as a measure of their swimming ability. Results show a concentration dependent effect of Zn on both species, leading to decreased swimming ability. Conversely, Fe exhibited varying effects on Paramecia and Euglena, with the latter displaying tolerance at lower concentrations but a notable response at higher concentrations. The advantage of the method is that owing to the non-imaging system, an enormous number of planktons can be processed. This allows for an immediate and statistically significant estimation of their swimming ability in response to environmental pollution.

Proceedings Article | 7 March 2023 Presentation + Paper

Fast and reliable micro-bioassay technique based on biospeckle

Arti Devi, Hirofumi Kadono, Uma Maheswari Rajagopalan

Proceedings Volume 12377, 123770F (2023) https://doi.org/10.1117/12.2651049

KEYWORDS: Biospeckle, Toxicity, Microorganisms, Industrial chemicals, Speckle pattern, Microscopes, Imaging systems, Chemical analysis, CCD cameras, Statistical analysis

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Recently, the pollution caused by various hazardous chemicals has become a very serious problem. Currently, 200 million kinds of chemical substances are registered, and it is technically and costly very difficult or even impossible to analyse, identify each chemical individually and then evaluate their toxicity on the environment. On the other hand, bioassay has been getting a lot of attention where the toxicity of environmental toxicity is assessed based on the reaction of micro-organisms such as plankton without identifying each chemical individually. For this technique, a microscope observation is required to obtain critical features such as alive/dead status and swimming ability. With smaller microorganisms, microscope observation becomes more difficult due to the narrower focal depth of the imaging system. In our study, to overcome these difficulties, we proposed a novel technique for the micro-bioassay utilizing laser biospeckle in the diffraction field generated from plankton. Paramecium chilomonas of size 30-40 µm was exposed to different pH conditions from control 7.2 to gradually decreasing by 0.5 till 3.7. Results reveal the sensitivity of laser biospeckles in detecting the subtle changes in the swimming behavior, the health of the microbe with change in pH suggesting the potential for fast assessment the toxicity of an environment.

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