We report the performance of a simple method for making quantitative bioluminescence measurements of a point-like
source embedded in small animals. In this method, video reflectometry is first used to obtain an estimate of the in situ
optical properties of the tissue containing the bioluminescent source. A 2-dimensional image of the bioluminescence
signal emitted from the surface of the animal is then acquired with a CCD. Using the measured optical properties, and a
simple diffusion theory model, an inversion algorithm is applied to retrieve the source depth and power from a region of
interest of the bioluminescence images. Two major factors determine the accuracy of the reconstruction: tissue
heterogeneity and curvature of the imaged surface. The use of measured optical properties to characterize in situ tissue
surmounts, to a degree, the heterogeneity problem: post mortem data from rats show that the relative power can be
retrieved within a factor of 2 and frequently within 20 %, and the depth within 1.0 mm for implanted depths of 4-10
mm, when the curvature effects were eliminated. For depths shallower than 4 mm, the errors in the retrieved depth are
consistently larger.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.