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Feiyang Lu

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Proceedings Article | 4 March 2019 Presentation + Paper

Raman spectroscopic imaging with frequency modulation based spatially encoded light

Tianyu Yan, Feiyang Lu, Duofang Chen, Yonghua Zhan, Jimin Liang, Xueli Chen

Proceedings Volume 10890, 1089008 (2019) https://doi.org/10.1117/12.2507734

KEYWORDS: Raman spectroscopy, Modulation, Image restoration, Imaging spectroscopy, Spectroscopy, Frequency modulation, Computer programming, Data acquisition, Sensors, Image quality

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Raman spectroscopic imaging can provide three-dimensional data set of samples, including two-dimensional spatial image and one-dimensional Raman spectral data. Currently, three strategies can be used to achieve Raman spectroscopic imaging, including point scanning, line scanning, and wide-field illumination. Point scanning method provides the best resolution but has low imaging speed. On the contrary, wide-field illumination can image fast but provides lower spatial resolution. To integrate the advantages of two methods, a new strategy for large-field Raman spectroscopic imaging was proposed, which uses the frequency modulation based spatially encoded light as the excitation. In this method, millions of single beams simultaneously illuminate on the sample to act as the wide-field illumination. Each beam illuminates on different positions of the sample, whose intensity are modulated with different frequencies. Thus, each excitation beam has its own modulation frequency and the excited Raman signal will carry the modulation information. At the detection end, a single point detector was used to collect the time series Raman signals carrying the unique modulation information. Using the sparse reconstruction based on demodulation strategy, the Raman image can be recovered effectively. The feasibility of the method was verified with numerical simulations. The results showed that it is feasible to conduct Raman spectroscopic imaging with high-resolution and high speed under the illumination of frequency modulation based spatially encoded light and the detection of single-point detector.

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