We introduce an infrared metasurface sensor based on plasmonic surface-enhanced infrared absorption spectroscopy combined with immunoassay, which detects alpha-synuclein, an early structural biomarker protein for neurodegenerative diseases with clinical specificity and identifies its different structural species using their unique spectroscopic signals. Unprecedentedly, we augmented the sensor with a Deep Neural Network, enabling quantitative differentiation of alpha-synuclein aggregates. Capable of multiplexing and retrieving aggregate signatures from complex biomatrix, our sensor shows promise for neurodegenerative disease diagnosis, disease progression monitoring, and drug efficacy assessment.
Near-infrared photobiomodulation has been reported to have therapeutic effects in several murine Alzheimer’s disease models. As a translational institution we sought to confirm and elaborate on these findings in a blind experiment study following GLP principles. 5xFAD mice were subjected to 810 nm transcutaneous LED photobiomodulation from one to six months of age with 0 (sham), 6 or 600 mW/cm2 power density. The effects - including dependence on light power values - were investigated with a battery of memory tests (novel object recognition, Y-maze and Morris water maze) and immunohistochemical and biochemical analyses (including ABeta plaque burden, neuronal count and inflammatory markers).
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