An algorithm for photographic images processing of blood samples in its native state was developed to determine the concentration of erythrocytes, leukocytes and platelets without individual separate preparation of cells’ samples. Special "photo templates" were suggested to use in order to identify red blood cells. The effect of "highlighting" of leukocytes, which was found by authors, was used to increase the accuracy of this type of cells counting. Finally to raise the resolution of platelets from leukocytes the areas of their photo images were used, but not their sizes. It is shown that the accuracy of cells counting for native blood samples may be comparable with the accuracy of similar studies for smears. At the same time the proposed native blood analysis simplifies greatly the procedure of sample preparation in comparison to smear, permits to move from the detection of blood cells ratio to the determination of their concentrations in the sample.
A new approach of the criterion assignment for registration of erythrocyte agglutinates to instrumentally determine blood group type is suggested. The criterion is based on comparison of R and G components of RGB decomposition of microscopy digital image taken for the blood-serum mixture sample. For the chosen experimental conditions, the minimal size (area) of RBC agglutinate to be registered by the criterion suggested is estimated theoretically. The proposed method was tested experimentally on the example of monitoring agglutinates in flow. The encouraging experimental results were obtained for improvement of the resolving power of the method; the optimal experimental conditions were revealed for maximum resolution. Though the suggested method was realized for dynamic (flow) blood group determination, it could also be applied for diagnostics in a stationary environment. This approach increases the reliability of RBC agglutinates registration and, hence, blood group typing. The results may be used to develop the apparatus for automated determination of human blood group.
The correction of experimentally measured luminescence excitation spectra of the samples with ZnCdS nanoparticles using the algorithm based on Monte Carlo simulation of the transport of radiation through the sample was made. The studied samples differed in amount of scattering, packing density of nanoparticles and spectral dependence of scattering and absorption. It has been shown that the developed correction technique makes possible the more accurately determining of the position and the shape of excitation bands and also the detecting of features of the spectra in particular exciton excitation bands.
The experimental investigation of the possibility to use the correlation analysis to register the process of human
erythrocytes agglutination on the base of flow method and digital photographing was carried out. To increase the
resolving power of flow method added by correlation analysis different variants of mathematical processing of digital
liquid flows photographs were realized.
The program which calculates the process of light scattering by particles with the Gaussian size distribution by single
scattering approximation was written. This program was used to calculate the optical characteristics of light scattering by
an optically thin layer of the polystyrene spheres in water. These data were applied to compare two optical schemes,
which are used for spectroscopy of the scattering samples. First method uses a wide-angle photodetector and second -
uses an integral sphere. It was shown that in spectrophotometric experiments with such samples we must use an integral
sphere to obtain a true spectrum. This program was used to calculation the optical characteristics of CuO nanocomposite
to investigate
It is offered to use a method of depolarize spectroscopy under investigation of spectral dependences of samples containing a low-concentrated absorbing substance localized on the scattering centers. Experimental data confirm, that the method of depolarization spectroscopy, for samples of this type, is more effective than standard methods of registration.
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