SignificanceIndocyanine green-based dynamic contrast-enhanced fluorescence imaging (DCE-FI) can objectively assess bone perfusion intraoperatively. However, it is susceptible to motion artifact due to patients’ involuntary respiration and mechanical disturbance. Reducing motion artifacts would significantly improve DCE-FI for orthopedic surgical guidance.AimOur primary objective is to develop an automated correction method to reduce motion artifacts in DCE-FI and improve the accuracy of bone perfusion assessment.ApproachWe developed an automated motion correction approach based on frame-by-frame mutual information (MI) and validated the effectiveness of this approach in various phantom studies and patient images from 45 imaging sessions of fifteen amputees.ResultsThe MI-based correction reduced motion artifacts by 93% for mechanical disturbances and 76% for simulated respiration in phantom studies. Patient images show improved alignment, improved kinetic curves, and restored bone perfusion-related parameters with an average correction of 4.3 and 9.6 mm in x- and y-axes per session.ConclusionsThe automated MI-based motion correction was able to eliminate motion artifacts effectively and significantly improved the quantitative assessment of bone perfusion by DCE-FI.
Indocyanine green (ICG)-based dynamic contrast-enhanced fluorescence imaging (DCE-FI) can objectively assess bone perfusion intraoperatively. However, it is susceptible to motion artifacts due to patient’s involuntary respiration during the 4.5-minute DCE-FI data acquisition. An automated motion correction approach based on mutual information (MI) frame-by-frame was developed to overcome this problem. In this approach, MIs were calculated between the reference and the adjacent frame translated and the maximal MI corresponded to the optimal translation. The images obtained from eighteen amputation cases were utilized to validate the approach and the results show that this correction can significantly reduce the motion artifacts and can improve the accuracy of bone perfusion assessment.
ICG-based dynamic contrast-enhanced fluorescence imaging (DCE-FI) and intraoperative DCE- magnetic resonance imaging (MRI) have been carried out nearly simultaneously in three lower extremity bone infection cases to investigate the relationship between these two imaging modalities for assessing bone blood perfusion during open orthopedic surgeries. Time-intensity curves in the corresponding regions of interest of two modalities were derived for comparison. The results demonstrated that ICG-based DCE-FI has higher sensitivity to perfusion changes while DCE-MRI provides superior and supplemental depth-related perfusion information. Research applying the depth-related perfusion information derived from MRI to improve the overall analytic modeling of intraoperative DCE-FI is ongoing.
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