The nervous system in the gastrointestinal wall is an autonomic nervous system that is essential for intestinal peristalsis, mucous secretion, and blood flow regulation. The degeneration or deficiency may cause passage disorder or functional obstruction. Pathological diagnosis accompanied by invasive tissue collection is essential for diagnosing the presence of this nervous system in the gastrointestinal wall. Therefore, we focused on Raman spectroscopy as a non-invasive diagnostic method. The purpose of this study is to develop new diagnostic methods based on optical techniques for non-invasive detection of the nervous system in the gastrointestinal wall using Raman spectroscopy.
Photodynamic diagnosis and therapy (PDD and PDT) involve the use of photochemical reactions induced by light irradiation with a photosensitizer for the detection and treatment of cancer cells and tissues. 5-Aminolevukinic acid (5-ALA) is a clinically used drug for PDT (5-ALA-PDT) in glioblastoma and bladder cancer. However, the conventional 5-ALA-PDT using visible light sours is not effective for the deep portion of organs and tissues. In this study, to overcome the limitation in penetration depth of 5-ALA-PDT, we developed two-photon excited 5-ALA-PDT system with NIR fiber laser for the effective treatment of peritoneal dissemination in gastric cancer.
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