This seminar course covers pioneering physics research in optics, astronomy, and nuclear physics, with an emphasis on discoveries by women physicists, African- American physicists, and other groups who are underrepresented in physics.
This first-year seminar course covers the history of modern physics using biomedical imaging as an overarching theme, focusing on both the scientists who made key discoveries and those unrecognized for their work.
This paper presents an intermediate-level undergraduate course on the physical principles of biomedical optics and imaging. Through in-class labs, Mathematica simulations, field trips, and group presentations, students learn about fundamental imaging concepts in optical microscopes. After developing an understanding of the role of the Fourier transform in image formation, the course shifts to non-optical imaging, including x-ray computed tomography, ultrasound, and magnetic resonance imaging. The significance of this course is its handson nature, and this paper offers examples of laboratory exercises and simulations to promote active learning in the classroom.
KEYWORDS: In vivo imaging, Luminescence, Brain, Microscopy, Neuroimaging, Multiphoton microscopy, Deep tissue imaging, Scattering, Signal attenuation, Blood
As a result of the large difference between scattering mean free paths and absorption lengths in brain tissue, scattering
dominates over absorption by water and intrinsic molecules in determining the attenuation factor for wavelengths
between 350 nm and 1300 nm. We propose using longer wavelengths for two-photon excitation, specifically the 1300-nm region, in order to reduce the effect of scattering and thereby increase imaging depth. We present two photon
fluorescence microscopy images of cortical vasculature in in vivo mouse brain beyond 1 mm. We also explore the
capabilities of the 1300-nm excitation for third harmonic generation microscopy of red blood cells in in vivo mouse
brain.
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