Prof. Peter T. C. So Profile

Prof. Peter T. C. So

Professor at Massachusetts Institute of Technology

SPIE Involvement:

Conference Program Committee | Conference Chair | Author | Editor | Instructor

Publications (115)

Proceedings Article | 11 October 2024 Open Access

3D nanofabrication of multi-functional optical/multi-functional metamaterials (Withdrawal Notice)

Gaojie Yang, Quansan Yang, Cheng Zheng, Peter So, Edward Boyden

Proceedings Volume PC12412, PC124120N (2024) https://doi.org/10.1117/12.2649532

KEYWORDS: Optical metamaterials, Nanofabrication, Optics manufacturing, Manufacturing, Lasers, Laser manufacturing, Laser applications

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SPIE Journal Paper | 3 April 2024 Open Access

Swept-source Raman spectroscopy of chemical and biological materials

Jeonggeun Song, Peter T. So, Hongki Yoo, Jeon Woong Kang

JBO, Vol. 29, Issue S2, S22703, (April 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.S2.S22703

KEYWORDS: Raman spectroscopy, Spectroscopy, Biological samples, Bandpass filters, Tissues, Tunable filters, Spectral resolution, Signal processing, Optical filters, Glucose

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SignificanceRaman spectroscopy has been used as a powerful tool for chemical analysis, enabling the noninvasive acquisition of molecular fingerprints from various samples. Raman spectroscopy has proven to be valuable in numerous fields, including pharmaceutical, materials science, and biomedicine. Active research and development efforts are currently underway to bring this analytical instrument into the field, enabling in situ Raman measurements for a wider range of applications. Dispersive Raman spectroscopy using a fixed, narrowband source is a common method for acquiring Raman spectra. However, dispersive Raman spectroscopy requires a bulky spectrometer, which limits its field applicability. Therefore, there has been a tremendous need to develop a portable and sensitive Raman system.AimWe developed a compact swept-source Raman (SS-Raman) spectroscopy system and proposed a signal processing method to mitigate hardware limitations. We demonstrated the capabilities of the SS-Raman spectroscopy by acquiring Raman spectra from both chemical and biological samples. These spectra were then compared with Raman spectra obtained using a conventional dispersive Raman spectroscopy system.ApproachThe SS-Raman spectroscopy system used a wavelength-swept source laser (822 to 842 nm), a bandpass filter with a bandwidth of 1.5 nm, and a low-noise silicon photoreceiver. Raman spectra were acquired from various chemical samples, including phenylalanine, hydroxyapatite, glucose, and acetaminophen. A comparative analysis with the conventional dispersive Raman spectroscopy was conducted by calculating the correlation coefficients between the spectra from the SS-Raman spectroscopy and those from the conventional system. Furthermore, Raman mapping was obtained from cross-sections of swine tissue, demonstrating the applicability of the SS-Raman spectroscopy in biological samples.ResultsWe developed a compact SS-Raman system and validated its performance by acquiring Raman spectra from both chemical and biological materials. Our straightforward signal processing method enhanced the quality of the Raman spectra without incurring high costs. Raman spectra in the range of 900 to 1200 cm−1 were observed for phenylalanine, hydroxyapatite, glucose, and acetaminophen. The results were validated with correlation coefficients of 0.88, 0.84, 0.87, and 0.73, respectively, compared with those obtained from dispersive Raman spectroscopy. Furthermore, we performed scans across the cross-section of swine tissue to generate a biological tissue mapping plot, providing information about the composition of swine tissue.ConclusionsWe demonstrate the capabilities of the proposed compact SS-Raman spectroscopy system by obtaining Raman spectra of chemical and biological materials, utilizing straightforward signal processing. We anticipate that the SS-Raman spectroscopy will be utilized in various fields, including biomedical and chemical applications.

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Proceedings Article | 13 March 2024 Presentation

High-speed computational multiphoton imaging through scattering media

Jian Zhao, Udith Haputhanthri, Mithunjha Anandakumar, Cheng Zheng, Feng-Chun Hsu, Brandon Weissbourd, Kendyll Burnell, Elly Nedivi, Dushan Wadduwage, Peter T. So

Proceedings Volume PC12847, PC1284709 (2024) https://doi.org/10.1117/12.3002076

KEYWORDS: Biological imaging, Scattering media, Silicon photomultipliers, Cameras, Microscopes, Image restoration, Electron multiplying charge coupled devices, Temporal resolution, Stereoscopy, Resolution enhancement technologies

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Proceedings Article | 13 March 2024 Presentation

High-throughput Raman spectroscopy by horizontally shifted collection fibers

Koseki Kobayashi-Kirschvink, Alex Matlock, Peter T. So, Jeon Woong Kang

Proceedings Volume PC12839, PC1283909 (2024) https://doi.org/10.1117/12.3008989

KEYWORDS: Raman spectroscopy, Spectrographs, Optical fibers, Biomedical applications, Urea, Spectrometers, Photons, Overlay metrology, Optical components, Optical alignment

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Proceedings Article | 13 March 2024 Presentation

Large field-of-view 3D computational phase imaging using differential structured illumination microscopy (dSIM)

Alex Matlock, Zahid Yaqoob, Peter T. So

Proceedings Volume PC12857, PC128570H (2024) https://doi.org/10.1117/12.3001613

KEYWORDS: Light sources and illumination, 3D modeling, 3D image processing, Phase imaging, Microscopy, Scattering, Super resolution, Spatial frequencies, Microscopes, Fluorophores

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Proceedings Article | 13 March 2024 Presentation

Combining Raman spectroscopy and holo-tomography to advance phenotyping of human cancer cells

Arianna Bresci, Renzo Vanna, Giulio Cerullo, Peter T. So, Dario Polli, Jeon Woong Kang

Proceedings Volume PC12834, PC128340G (2024) https://doi.org/10.1117/12.3001665

KEYWORDS: Raman spectroscopy, Cell phenotyping, Cancer, Image resolution, Tomography, Refractive index, Microscopy, Medical research, Hyperspectral imaging, Diseases and disorders

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Proceedings Article | 13 March 2024 Presentation

Single-shot full-field reflection-mode off-axis quantitative phase microscopy with temporal focusing

Yuechuan Lin, Peter So, Zahid Yaqoob

Proceedings Volume PC12852, PC128520D (2024) https://doi.org/10.1117/12.3000099

KEYWORDS: Reflection, Microscopy, Biological imaging, Phase imaging, Multiplexing, Light sources and illumination, Ultrafast imaging, Transmittance, Tissues, Tissue optics

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Proceedings Article | 13 March 2024 Presentation

Label-free identification of biochemical variations in brain organoid maturation stages through Raman spectroscopy

Giulia Bruno, Koseki J. Kobayashi-Kirschvink, Michal Lipinski, Christian Tentellino, Peter T. So, Paola Arlotta, Jeon Woong Kang, Francesco De Angelis

Proceedings Volume PC12854, PC128540Y (2024) https://doi.org/10.1117/12.3001590

KEYWORDS: Brain, Raman spectroscopy, Random forests, Proteins, Nondestructive evaluation, Neurological disorders, Microscopes, Machine learning, Laser sources, In vitro testing

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Proceedings Article | 12 March 2024 Presentation + Paper

Multiphoton imaging of the formation of fluorescent advanced glycation end products in tissues

Sohidul Mondal, Chih-Ju Lin, Nasim Kamely, Sheng-Lin Lee, Jeon-Woong Kang, Peter T. So, Chen Yuan Dong

Proceedings Volume 12847, 1284708 (2024) https://doi.org/10.1117/12.2691192

KEYWORDS: Tissues, Second harmonic generation, Autofluorescence, Glucose, Collagen, Blood, Cornea, Aorta, Physics, Transform theory

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Proceedings Article | 12 August 2023 Presentation

Quantitative phase microscopy for biomaterial and device characterization

Peter T. So

Proceedings Volume PC12622, PC1262203 (2023) https://doi.org/10.1117/12.2676493

KEYWORDS: Microscopy, Microfluidics, Liquid crystals, Control systems, Tissues, Refractive index, Reflection, Process control, Polarization imaging, Phase imaging

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Proceedings Article | 17 March 2023 Presentation

In situ process monitoring of two-photon lithography with optical diffraction tomography

Cheng Zheng, Peter So

Proceedings Volume PC12412, PC124120L (2023) https://doi.org/10.1117/12.2649490

KEYWORDS: Lithography, Tomography, Optical tomography, Diffraction, 3D modeling, Visualization, Structural design, Statistical modeling, Scattering, Scanning electron microscopy

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Proceedings Article | 16 March 2023 Presentation

Noninvasive glucose monitoring using Raman spectroscopy (Conference Presentation)

Jeon Woong Kang, Hyung Min Kim, Hong Man Yoon, Luis Galindo, Peter So

Proceedings Volume PC12387, PC1238702 (2023) https://doi.org/10.1117/12.2647397

KEYWORDS: Glucose, Raman spectroscopy, Blood, Molecules, Skin, Sensors, Molecular spectroscopy, In vivo imaging, Diagnostics, Adhesives

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Proceedings Article | 16 March 2023 Presentation

Acoustic-driven quantitative phase microscope for quantifying cell mechanical properties at sub-cellular level (Conference Presentation)

Yuechuan Lin, Xiang Zhang, Rebecca Zubajlo, Zahid Yaqoob, Brian Anthony, Peter T. So

Proceedings Volume PC12389, PC123890H (2023) https://doi.org/10.1117/12.2648478

KEYWORDS: Microscopes, Acoustics, Phase shift keying, Microscopy, Cell mechanics, Ultrasonography, Tissues, Speckle, Spatial resolution, Spatial coherence

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Proceedings Article | 16 March 2023 Presentation

Interferometric phase and absorption microscopy (iPAM) for sickle cell disease imaging (Conference Presentation)

Alex Matlock, Yuhao Qiang, Ming Dao, John Higgins, Zahid Yaqoob, Peter So

Proceedings Volume PC12389, PC123890R (2023) https://doi.org/10.1117/12.2648428

KEYWORDS: Single crystal X-ray diffraction, Phase interferometry, Microscopy, Interferometry, Absorption, Blood, Proteins, Polymers, Molecules, Microfluidics

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Proceedings Article | 16 March 2023 Presentation

Towards fabricating visible wavelength D2NNs through multi-level quantization for quantitative phase imaging (Conference Presentation)

Hasindu Kariyawasam, Ramith Hettiarachchi, Quansan Yang, Udith Haputhanthri, Kithmini Herath, Chamira Edussooriya, Edward Boyden, Peter So, Dushan Wadduwage

Proceedings Volume PC12389, PC123890C (2023) https://doi.org/10.1117/12.2651937

KEYWORDS: Quantization, Phase imaging, Microscopy, Fabrication, Process modeling, Neural networks, Machine learning, Chemical elements, Terahertz radiation, Object recognition

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Proceedings Article | 16 March 2023 Presentation

Encoding light for high-through 3D tissue imaging (Conference Presentation)

Peter T. So, Dushan Wadduwage

Proceedings Volume PC12393, PC1239309 (2023) https://doi.org/10.1117/12.2655637

KEYWORDS: 3D image processing, Tissue optics, Stereoscopy, Computer programming, Tissues, Structured light, Scattering, Photons, Optical lithography, In vivo imaging

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Proceedings Article | 15 March 2023 Presentation

Differential structured illumination microscopy for 3D computational phase imaging (Conference Presentation)

Alex Matlock, Zahid Yaqoob, Peter T. So

Proceedings Volume PC12385, PC123850F (2023) https://doi.org/10.1117/12.2647861

KEYWORDS: 3D image processing, Phase imaging, Microscopy, Resolution enhancement technologies, Luminescence, 3D modeling, Stereoscopy, Spatial frequencies, Imaging systems, Image processing

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Proceedings Article | 15 March 2023 Presentation

Raman2RNA: Live-cell label-free prediction of single-cell expression profiles by Raman microscopy (Conference Presentation)

Koseki Kobayashi-Kirschvink, Charles Comiter, Ke Zhang, Jeon Woong Kang, Jian Shu, Peter So, Aviv Regev

Proceedings Volume PC12383, PC1238309 (2023) https://doi.org/10.1117/12.2649072

KEYWORDS: Raman spectroscopy, Microscopy, Stem cells, Profiling, Organisms, In vivo imaging, In vitro testing

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Proceedings Article | 7 March 2022 Presentation

DEEP learning powered De-scattering with Excitation Patterning (DEEP) for high-throughput wide-field multiphoton microscopy

Navodini Wijethilake, Cheng Zheng, Jong Park, Murat Yildirim, Udith Haputhanthri, Peter T. So, Dushan Wadduwage

Proceedings Volume PC11965, PC119650B (2022) https://doi.org/10.1117/12.2615331

KEYWORDS: Optical lithography, Multiphoton microscopy, Light scattering, Mathematical modeling, Data modeling, Luminescence, Image quality, Tissues, Scattering, Inverse problems

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Proceedings Article | 25 March 2021 Presentation + Paper

Multiphoton imaging of the fluorescent advanced glycation end products in tissues

Chih-Ju Lin, Jeon Woong Kang, Peter. T. C. So, Chen-Yuan Dong

Proceedings Volume 11648, 1164816 (2021) https://doi.org/10.1117/12.2583608

KEYWORDS: Tissues, Skin, Proteins, Multiphoton microscopy, Kidney, Glucose, Cornea, Aorta

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Proceedings Article | 25 March 2021 Presentation + Paper

Multiphoton imaging of autofluorescent advanced glycation end product formation in porcine aorta

Chih-Ju Lin, Jeon Woong Kang, Peter T. C. So, Chen-Yuan Dong

Proceedings Volume 11648, 1164815 (2021) https://doi.org/10.1117/12.2583607

KEYWORDS: Aorta, Tissues, Blood, Multiphoton microscopy, Diffusion

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Proceedings Article | 5 March 2021 Poster + Presentation

High throughput three-dimensional imaging cytometry based on single-shot optical diffraction tomography system

Yanping He, Baoliang Ge, Mo Deng, Yijin Wang, Md Habibur Rahman, Yi Ping Ho, Liting Duan, Zahid Yaqoob, George Barbastathis, Peter T. C. So, Renjie Zhou

Proceedings Volume 11654, 1165415 (2021) https://doi.org/10.1117/12.2579038

KEYWORDS: 3D image processing, Tomography, Optical tomography, Diffraction, Singular optics, Microfluidics, Algorithm development, Stem cells, Regenerative medicine, Refractive index

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Proceedings Article | 5 March 2021 Presentation

Welcome and Introduction to SPIE Conference 11648

Ammasi Periasamy, Peter T. So, Karsten König

Proceedings Volume 11648, 1164802 (2021) https://doi.org/10.1117/12.2592752

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Proceedings Article | 5 March 2021 Presentation

Ultrafast two-photon volumetric scanning microscopy

Chien-Sheng Wang, Yu-Hsuan Tsai, Po-Yuan Wang, Chih-Wei Liu, Chia-Fu Chou, Vijay Raj Singh, Peter T. So, Shi-Wei Chu

Proceedings Volume 11654, 116540F (2021) https://doi.org/10.1117/12.2582693

KEYWORDS: Brain, Ultrafast phenomena, Neurons, Microscopy, Scattering, Laser scattering, Two photon excitation microscopy, Tissues, Temporal resolution, Spatial resolution

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SPIE Journal Paper | 26 November 2020 Open Access

High spatial and temporal resolution synthetic aperture phase microscopy

Cheng Zheng, Di Jin, Yanping He, Hongtao Lin, Juejun Hu, Zahid Yaqoob, Peter T. So, Renjie Zhou

AP, Vol. 2, Issue 06, 065002, (November 2020) https://doi.org/10.1117/12.10.1117/1.AP.2.6.065002

KEYWORDS: Temporal resolution, Microscopy, Synthetic aperture microscopy, Video, Digital micromirror devices, Diffraction, Scanning electron microscopy, Objectives, Image resolution, Coherence imaging

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Proceedings Article | 11 March 2020 Presentation

High-speed optical diffraction tomography (ODT) with deep-learning approach (Conference Presentation)

Baoliang Ge, Mo Deng, George Barbastathis, Peter T. So, Renjie Zhou, Zahid Yaqoob

Proceedings Volume 11249, 1124906 (2020) https://doi.org/10.1117/12.2546172

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Proceedings Article | 11 March 2020 Presentation

Quantitative imaging of anisotropic dynamics enabled by polarized shearing interferometric microscopy (Conference Presentation)

Baoliang Ge, Qing Zhang, Renjie Zhou, Zahid Yaqoob, Irmgard Bischofberger, Peter T. So

Proceedings Volume 11249, 112490I (2020) https://doi.org/10.1117/12.2543538

KEYWORDS: Interferometry, Microscopy, Polarization, Polarized microscopy, Liquid crystals, Inspection, Imaging systems, Optical inspection, Visual analytics, Visualization

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Proceedings Article | 9 March 2020 Presentation

High-throughput three-dimensional imaging cytometer for subnuclear foci quantification (Conference Presentation)

Cheng Zheng, Dushan Wadduwage, Jong Park, Christy Chao, Jenny Kay, Norah Owiti, Zachary Nagel, Bevin Engelward, Peter T. So

Proceedings Volume 11243, 112430X (2020) https://doi.org/10.1117/12.2546302

KEYWORDS: 3D image processing, Double sideband modulation, Neural networks, Image resolution, Confocal microscopy, Cancer, Stereoscopy, Laser imaging, Microscopy, Luminescence

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Proceedings Article | 25 February 2020 Presentation + Paper

Analysis diffusion and glycation rate of artery in high concentration sugar condition via autofluorescence of advanced glycation end productions

Chih-Ju Lin, Jeon Woong Kang, Peter T. So, Chen-Yuan Dong

Proceedings Volume 11215, 112150D (2020) https://doi.org/10.1117/12.2545589

KEYWORDS: Aorta, Arteries, Tissues, Diffusion, Harmonic generation, Glucose, Pathogens, Collagen, Multiphoton fluorescence microscopy, Multiphoton microscopy, Second-harmonic generation

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Proceedings Article | 17 February 2020 Presentation + Paper

Multiphoton autofluorescence imaging of advanced glycation end products in glycated tissues

Chih-Ju Lin, Jeon Woong Kang, Peter T. So, Chen-Yuan Dong

Proceedings Volume 11244, 112441V (2020) https://doi.org/10.1117/12.2545548

KEYWORDS: Tissues, Cornea, Collagen, Auto-fluorescence imaging, Aorta, Pathogens, Second-harmonic generation, Glucose, Multiphoton fluorescence microscopy, Multiphoton microscopy, Skin, Eye

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SPIE Journal Paper | 31 January 2020 Open Access

Special Section Guest Editorial: Thirty Years of Multiphoton Microscopy in the Biomedical Sciences

Ammasi Periasamy, Karsten König, Peter So

JBO, Vol. 25, Issue 01, 014501, (January 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.1.014501

KEYWORDS: Multiphoton microscopy, Biomedical optics, Microscopes, Luminescence, Microscopy, Tissues, Absorption, Confocal microscopy, Imaging systems, Infrared imaging

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Proceedings Article | 4 March 2019 Presentation

Studying nucleic membrane fluctuations of tumor cells treated with chemotherapeutic drug using confocal reflectance quantitative phase microscopy (Conference Presentation)

Vijay Raj Singh, Zahid Yaqoob, Peter T. So

Proceedings Volume 10887, 108871G (2019) https://doi.org/10.1117/12.2513575

KEYWORDS: Confocal microscopy, Tumors, Reflectivity, Microscopy, Spectroscopy, Temporal resolution, Lung cancer, Cancer

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Proceedings Article | 4 March 2019 Presentation

The application of interferometric imaging in quantitative mechanobiology (Conference Presentation)

Peter T. So

Proceedings Volume 10880, 108800V (2019) https://doi.org/10.1117/12.2516343

KEYWORDS: Interferometry, Microscopy, Tissues, Spectral resolution, Resolution enhancement technologies, Optical imaging, Biomedical optics, Blood, Cancer, Mechanics

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Proceedings Article | 4 March 2019 Presentation

Two-photon focal modulation microscopy for deep tissue imaging (Conference Presentation)

Rui Chen, Younghoon Shin, E. Du, Shuhao Shen, Peter T. So, Nanguang Chen

Proceedings Volume 10882, 108821D (2019) https://doi.org/10.1117/12.2509584

KEYWORDS: Modulation, Microscopy, Deep tissue imaging, Phase shift keying, Tissues, Phase only filters, Two photon excitation microscopy, Scattering, Spatial resolution, Modulators

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Proceedings Article | 4 March 2019 Presentation

Low-coherent optical diffraction tomography by angle-scanning illumination (Conference Presentation)

KyeoReh Lee, Seungwoo Shin, Zahid Yaqoob, Peter T. So, YongKeun Park

Proceedings Volume 10887, 108870A (2019) https://doi.org/10.1117/12.2513188

KEYWORDS: Diffraction, Optical tomography, Tomography, Phase imaging, Interferometry, Microscopy, Coherence imaging, Imaging systems, Light sources, Interferometers

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In coherent imaging systems, parasitic fringes and concentric patterns could commonly be found due to the unwanted multiple reflections [1]. One fundamental solution for the coherent noise is to use a temporally incoherent light source. However, maintaining the full-field interference fringe contrast using temporally incoherent light is not straightforward for interferometric imaging techniques such as quantitative phase imaging (QPI). Fortunately, several brilliant incoherent QPI techniques have been realized for wide-field imaging mainly through the common-path interferometer geometries [2-4]. However, it has been more difficult to implement incoherent-light-based optical diffraction tomography (ODT) due to the additional angle-scanning illumination unit that induces severe decoherence over the camera field-of-view [5]. Here, we suggest a temporally low-coherence optical diffraction tomography by angle-scanning broadband illumination based on general Mach-Zehnder interferometric geometry. We have designed an angle-scanning unit composed of two digital micromirror devices (DMDs) to maintain interference fringe contrast across the whole field of view during the angle-scanning sequence. Further, we have developed the theoretical framework for ODT reconstruction using incoherent light. In the light of our recent developments, we will discuss the theoretical and practical constraints, and suggest the best degree of incoherency for incoherent ODT. We will also demonstrate the incoherent optical diffraction tomography of plastic microspheres, human blood cells and rat pheochromocytoma cells. References 1. I. Choi, K. Lee, and Y. Park, "Compensation of aberration in quantitative phase imaging using lateral shifting and spiral phase integration," Opt. Express 25, 30771-30779 (2017). 2. Z. Wang, L. Millet, M. Mir, H. Ding, S. Unarunotai, J. Rogers, M. U. Gillette, and G. Popescu, "Spatial light interference microscopy (SLIM)," Opt. Express 19, 1016-1026 (2011). 3. B. Bhaduri, H. Pham, M. Mir, and G. Popescu, "Diffraction phase microscopy with white light," Opt. Lett. 37, 1094-1096 (2012). 4. Y. Baek, K. Lee, J. Yoon, K. Kim, and Y. Park, "White-light quantitative phase imaging unit," Opt. Express 24, 9308-9315 (2016). 5. M. Rinehart, Y. Zhu, and A. Wax, "Quantitative phase spectroscopy," Biomed. Opt. Express 3, 958-965 (2012).

Proceedings Article | 4 March 2019 Paper

Vibrating dichroic MEMS scanner based line scan multiphoton endomicroscope

Yingshun Xu, Jin Cheng, Naitao Xu, Lanping Zhu, Yuzhu Cao, Bei Fang, Xin Chen, Suogang Wang, Peter T. So

Proceedings Volume 10931, 1093116 (2019) https://doi.org/10.1117/12.2510040

KEYWORDS: Nonlinear optics, Endoscopes, Scanners, Microelectromechanical systems, Objectives, Microscopes, Mirrors, Deep reactive ion etching, Endoscopy, GRIN lenses

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Proceedings Article | 15 March 2018 Presentation

Investigation of depth-sectioning in broad-band dynamic speckle-field interferometric microscopy (Conference Presentation)

Sungsam Kang, Renjie Zhou, Zahid Yaqoob, Peter T. So

Proceedings Volume 10503, 105030C (2018) https://doi.org/10.1117/12.2290542

KEYWORDS: Interferometry, Microscopy, Optical design, Speckle interferometry, Phase imaging, In vivo imaging, 3D acquisition, Optical microscopy, Spatial resolution, Interferometers

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Proceedings Article | 15 March 2018 Presentation

A portable quantitative phase microscope (Conference Presentation)

Renjie Zhou, Di Jin, Zahid Yaqoob, Peter So

Proceedings Volume 10503, 105030D (2018) https://doi.org/10.1117/12.2289147

KEYWORDS: Digital holography, Microscopy, Real time imaging, Measurement devices, Imaging systems, Imaging devices, Microscopes, Phase imaging, 3D scanning, Profilometers

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Proceedings Article | 14 March 2018 Presentation

Label free assessment of ultra-violet radiation induced damages in skin cells (Conference Presentation)

Surya Singh, Sungsam Kang, Jeon Woong Kang, Peter T. So, Ramachandra Dasari, Zahid Yaqoob, Ishan Barman

Proceedings Volume 10467, 104670T (2018) https://doi.org/10.1117/12.2287516

KEYWORDS: Raman spectroscopy, Phase imaging, Ultraviolet radiation, Skin, Chemical analysis, Skin cancer, Molecules, Biological research, Composites, Cell mechanics

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Proceedings Article | 28 July 2017 Paper

A classification model for non-alcoholic steatohepatitis (NASH) using confocal Raman micro-spectroscopy

Jie Yan, Yang Yu, Jeon Woong Kang, Zhi Yang Tam, Shuoyu Xu, Eliza Li Shan Fong, Surya Pratap Singh, Ziwei Song, Lisa Tucker Kellogg, Peter So, Hanry Yu

Proceedings Volume 10411, 104110Y (2017) https://doi.org/10.1117/12.2281445

KEYWORDS: Raman spectroscopy, Confocal microscopy, Liver, Biological research

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Proceedings Article | 3 May 2017 Presentation

DMD based optical diffraction tomography (Conference Presentation)

Di Jin, Renjie Zhou, Zahid Yaqoob, Peter T. So

Proceedings Volume 10117, 101170C (2017) https://doi.org/10.1117/12.2249238

KEYWORDS: Beam propagation method, Interferometry, Tomography, Diffraction, Digital micromirror devices, Holograms, Real time imaging, Optical tomography, Microscopy, Reconstruction algorithms

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Proceedings Article | 24 April 2017 Presentation

Confocal reflectance quantitative phase microscope system for cellular membranes dynamics study (Conference Presentation)

Vijay Raj Singh, Zahid Yaqoob, Peter T. So

Proceedings Volume 10074, 100740I (2017) https://doi.org/10.1117/12.2252350

KEYWORDS: Imaging systems, Mechanics, Confocal microscopy, Microscopes, Reflectivity, Temporal resolution, Plasma, Microscopy, Micromirrors, Biomedical optics

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Proceedings Article | 24 April 2017 Presentation

Solving the inverse scattering problem in reflection-mode dynamic speckle-field phase microscopy (Conference Presentation)

Renjie Zhou, Peter T. So, Zahid Yaqoob, Di Jin, Poorya Hosseini, Cuifang Kuang, Vijay Raj Singh, Yang-Hyo Kim, Ramachandra Dasari

Proceedings Volume 10074, 100741C (2017) https://doi.org/10.1117/12.2251378

KEYWORDS: 3D metrology, Imaging systems, Microscopy, Tomography, Diffraction, 3D modeling, Stereoscopy, 3D image processing, Inverse scattering problem, Optical tomography

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Proceedings Article | 24 April 2017 Presentation

High-speed and high-resolution quantitative phase imaging with digital-micromirror device-based illumination (Conference Presentation)

Renjie Zhou, Di Jin, Zahid Yaqoob, Peter T. So

Proceedings Volume 10074, 100740C (2017) https://doi.org/10.1117/12.2256130

KEYWORDS: Phase imaging, Synthetic aperture radar, Digital micromirror devices, Imaging systems, Microscopy, Algorithm development, Cameras, Mirrors, Optical lithography, Biomedical optics

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Proceedings Article | 24 April 2017 Open Access

Third harmonic generation imaging of intact human cerebral organoids to assess key components of early neurogenesis in Rett Syndrome (Conference Presentation)

Murat Yildirim, Danielle Feldman, Tianyu Wang, Dimitre Ouzounov, Stephanie Chou, Justin Swaney, Kwanghun Chung, Chris Xu, Peter T. So, Mriganka Sur

Proceedings Volume 10069, 100690A (2017) https://doi.org/10.1117/12.2256182

KEYWORDS: Brain, Optical lithography, Stem cells, Neurogenesis, Harmonic generation, Microscopy, Proteins, 3D modeling, Pulsed laser operation, Computer programming

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Proceedings Article | 18 April 2017 Paper

Cellular biophysical markers of hydroxyurea treatment in sickle cell disease

Peter T. So, Poorya Hosseini, Sabia Abidi, E. Du, Dimitrios Papageorgiou, YongKeun Park, John Higgins, Gregory Kato, Subra Suresh, Ming Dao, Zahid Yaqoob

Proceedings Volume 10251, 1025103 (2017) https://doi.org/10.1117/12.2273139

KEYWORDS: Blood, Single crystal X-ray diffraction, Interferometry, Wavefronts, Mechanical engineering, Materials science, Fetus, Blood circulation, Oxygen, Laser applications

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Proceedings Article | 27 April 2016 Presentation

Confocal reflectance quantitative phase microscopy system for cell biology studies (Conference Presentation)

Vijay Raj Singh, Peter T. So

Proceedings Volume 9718, 97182U (2016) https://doi.org/10.1117/12.2217962

KEYWORDS: Confocal microscopy, Microscopy, Reflectivity, Cell biology, Refractive index, Micromirrors, Active optics, Biomedical optics, Optical testing, Mirrors

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Proceedings Article | 27 April 2016 Presentation

Multi-color phase imaging and sickle cell anemia (Conference Presentation)

Poorya Hosseini, Renjie Zhou, Zahid Yaqoob, Peter T. So

Proceedings Volume 9718, 97182E (2016) https://doi.org/10.1117/12.2217321

KEYWORDS: Phase imaging, Blood, Spectral resolution, Interferometry, Microscopy, Phase measurement, Tomography, Refractive index, Microscopes, Spectroscopy

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Quantitative phase measurements at multiple wavelengths has created an opportunity for exploring new avenues in phase microscopy such as enhancing imaging-depth (1), measuring hemoglobin concentrations in erythrocytes (2), and more recently in tomographic mapping of the refractive index of live cells (3). To this end, quantitative phase imaging has been demonstrated both at few selected spectral points as well as with high spectral resolution (4,5). However, most of these developed techniques compromise imaging speed, field of view, or the spectral resolution to perform interferometric measurements at multiple colors. In the specific application of quantitative phase in studying blood diseases and red blood cells, current techniques lack the required sensitivity to quantify biological properties of interest at individual cell level. Recently, we have set out to develop a stable quantitative interferometric microscope allowing for measurements of such properties for red cells without compromising field of view or speed of the measurements. The feasibility of the approach will be initially demonstrated in measuring dispersion curves of known solutions, followed by measuring biological properties of red cells in sickle cell anemia. References: 1. Mann CJ, Bingham PR, Paquit VC, Tobin KW. Quantitative phase imaging by three-wavelength digital holography. Opt Express. 2008;16(13):9753–64. 2. Park Y, Yamauchi T, Choi W, Dasari R, Feld MS. Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. Opt Lett. 2009;34(23):3668–70. 3. Hosseini P, Sung Y, Choi Y, Lue N, Yaqoob Z, So P. Scanning color optical tomography (SCOT). Opt Express. 2015;23(15):19752–62. 4. Jung J-H, Jang J, Park Y. Spectro-refractometry of individual microscopic objects using swept-source quantitative phase imaging. Anal Chem. 2013;85(21):10519–25. 5. Rinehart M, Zhu Y, Wax A. Quantitative phase spectroscopy. Biomed Opt Express. 2012;3(5):958–65.

Proceedings Article | 27 April 2016 Presentation

High-sensitivity quantitative phase microcopy (Conference Presentation)

Renjie Zhou, Cuifang Kuang, Poorya Hosseini, Ravi Chowdhary, Zahid Yaqoob, Peter T. So

Proceedings Volume 9718, 97182N (2016) https://doi.org/10.1117/12.2212849

KEYWORDS: Cameras, Microscopy, Microscopes, Imaging systems, Optical filters, Electronic filtering, Scattering, Photodetectors, Phase contrast, Phase imaging

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Proceedings Article | 27 April 2016 Open Access

Depth-resolved incoherent and coherent wide-field high-content imaging (Conference Presentation)

Peter So

Proceedings Volume 9712, 971205 (2016) https://doi.org/10.1117/12.2217744

KEYWORDS: Structured light, Coherence imaging, Biology, Medicine, Nonlinear optics, Spectroscopy, Information fusion, Interferometers, Toxicology, Cancer

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Proceedings Article | 14 July 2015 Paper

Subnuclear foci quantification using high-throughput 3D image cytometry

Dushan Wadduwage, Marcus Parrish, Heejin Choi, Bevin Engelward, Paul Matsudaira, Peter T. So

Proceedings Volume 9536, 953607 (2015) https://doi.org/10.1117/12.2185005

KEYWORDS: Image segmentation, 3D image processing, Double sideband modulation, Image processing, Image processing algorithms and systems, Mirrors, Ionizing radiation, Proteins, Visualization, Image analysis

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SPIE Journal Paper | 6 April 2015 Open Access

Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs

Christopher Rowlands, Oliver Bruns, Moungi Bawendi, Peter T. So

JBO, Vol. 20, Issue 06, 061107, (April 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.6.061107

KEYWORDS: Spatial frequencies, Microscopy, Modulation transfer functions, Tissues, Two photon excitation microscopy, Microscopes, Multiphoton microscopy, Objectives, Image resolution, Photons

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Proceedings Article | 5 March 2015 Paper

Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control

Yi Xue, Christopher Rowlands, Peter So

Proceedings Volume 9329, 932927 (2015) https://doi.org/10.1117/12.2078509

KEYWORDS: Microscopy, Spatial light modulators, Super resolution, Two photon imaging, 3D image processing, Luminescence, Imaging systems, Phase retrieval, Multiphoton microscopy, Fourier transforms

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SPIE Journal Paper | 11 November 2014 Open Access

In vivo label-free quantification of liver microcirculation using dual-modality microscopy

Jie Yan, Yuzhan Kang, Shuoyu Xu, Lee-Ling Ong, Shuangmu Zhuo, Ralph Bunte, Nanguang Chen, H. Harry Asada, Peter T. So, Ian Wanless, Hanry Yu

JBO, Vol. 19, Issue 11, 116006, (November 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.11.116006

KEYWORDS: Liver, In vivo imaging, Microscopy, Speckle, Luminescence, Laser speckle contrast imaging, Blood, Tissues, Image filtering, Medical research

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Proceedings Article | 5 March 2014 Paper

Quantifying the surface chemistry of 3D matrices in situ

Dimitrios Tzeranis, Peter T. So, Ioannis Yannas

Proceedings Volume 8956, 89560L (2014) https://doi.org/10.1117/12.2038664

KEYWORDS: Collagen, Chemistry, Receptors, Matrices, Molecules, Signal detection, Natural surfaces, Tissues, Proteins, Physiology

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Proceedings Article | 4 March 2014 Paper

Image informatics for studying signal transduction in cells interacting with 3D matrices

Dimitrios Tzeranis, Jin Guo, Chengpin Chen, Ioannis Yannas, Xunbin Wei, Peter T. So

Proceedings Volume 8947, 89471R (2014) https://doi.org/10.1117/12.2038677

KEYWORDS: Information science, 3D image processing, Image processing, Proteins, Imaging systems, Matrices, Image segmentation, Collagen, Mahalanobis distance, Microscopy

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SPIE Journal Paper | 5 April 2013 Open Access

Special Section Guest Editorial: Multiphoton Microscopy: Technical Innovations, Biological Applications, and Clinical Diagnostics

Chen-Yuan Dong, Peter So, Jerome Mertz, Karsten Koenig, Chris Xu, Paul Campagnola

JBO, Vol. 18, Issue 03, 031101, (April 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.3.031101

KEYWORDS: Multiphoton microscopy, Diagnostics, Optical microscopy, Harmonic generation, Tissues, Microscopy, Molecules, Absorption, Range imaging, Tissue optics

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SPIE Journal Paper | 19 March 2012 Open Access

Methodological considerations for global analysis of cellular FLIM/FRET measurements

Nur Aida Abdul Rahim, Roger Kamm, Peter So, Serge Pelet

JBO, Vol. 17, Issue 02, 026013, (March 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.2.026013

KEYWORDS: Fluorescence resonance energy transfer, Infrared imaging, Photon counting, Proteins, Luminescence, Fluorescence lifetime imaging, Image segmentation, Biological research, Signal to noise ratio, Data modeling

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Proceedings Article | 11 February 2012 Paper

Self-referenced quantitative phase microscopy

Timothy Hillman, Niyom Lue, Yongjin Sung, Ramachandra Dasari, Peter So, Zahid Yaqoob

Proceedings Volume 8227, 82271S (2012) https://doi.org/10.1117/12.909701

KEYWORDS: Microscopy, Holograms, Phase imaging, Diffraction, Microscopes, Digital holography, Phase measurement, Mirrors, Prisms, Statistical analysis

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Proceedings Article | 28 February 2011 Paper

Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

Elijah Y. Yew, Heejin Choi, Daekeun Kim, Peter T. So

Proceedings Volume 7903, 79031O (2011) https://doi.org/10.1117/12.876068

KEYWORDS: Microscopes, Image processing, Two photon excitation microscopy, Microscopy, Absorption, Multiphoton microscopy, Objectives, Luminescence, Diffraction gratings, Imaging systems

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Proceedings Article | 12 February 2011 Paper

Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima

Naveen Balla, Colin Sheppard, Peter So

Proceedings Volume 7910, 79100N (2011) https://doi.org/10.1117/12.876014

KEYWORDS: Gold, Luminescence, Absorption, Femtosecond phenomena, Near infrared, Nanoparticles, Particles, Plasmons, Deep tissue imaging, Nanorods

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Proceedings Article | 11 February 2011 Paper

Intensity normalization of two-photon microscopy images for liver fibrosis analysis

Vijay Raj Singh, Jagath Rajapakse, Hanry Yu, Peter T. So

Proceedings Volume 7903, 79030P (2011) https://doi.org/10.1117/12.876246

KEYWORDS: Image segmentation, Image processing, Liver, Two photon excitation microscopy, Microscopes, Second-harmonic generation, Image analysis, Collagen, Imaging arrays, Tissues

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SPIE Journal Paper | 1 September 2010 Open Access

Toward surface quantification of liver fibrosis progression

Yuting He, Chiang Huen Kang, Shuoyu Xu, Xiaoye Tuo, Scott Trasti, Dean Tai, Anju Raja, Qiwen Peng, Peter So, Jagath Rajapakse, Roy Welsch, Hanry Yu

JBO, Vol. 15, Issue 05, 056007, (September 2010) https://doi.org/10.1117/12.10.1117/1.3490414

KEYWORDS: Liver, Collagen, Natural surfaces, Tissues, Second-harmonic generation, Biopsy, Image segmentation, Feature extraction, Nanotechnology, Binary data

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SPIE Journal Paper | 1 September 2010 Open Access

Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

Anju Raja, Shuoyu Xu, Wanxin Sun, Jianbiao Zhou, Dean Tai, Chien-Shing Chen, Jagath Rajapakse, Peter So, Hanry Yu

JBO, Vol. 15, Issue 05, 056016, (September 2010) https://doi.org/10.1117/12.10.1117/1.3497565

KEYWORDS: Collagen, Tumors, Scanning helium ion microscopy, Microscopes, Second-harmonic generation, Signal to noise ratio, Visualization, Tissues, Modulation, Bragg cells

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SPIE Journal Paper | 1 July 2010 Open Access

Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy

Jae-Won Cha, Jérôme Ballesta, Peter T. So

JBO, Vol. 15, Issue 04, 046022, (July 2010) https://doi.org/10.1117/12.10.1117/1.3475954

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SPIE Journal Paper | 1 May 2010 Open Access

Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy

Tzu-Lin Sun, Yuan Liu, Ming-Chin Sung, Hsiao-Ching Chen, Chun-Hui Yang, Vladimir Hovhannisyan, Wei-Chou Lin, Yung-Ming Jeng, Wei-Liang Chen, Ling-Ling Chiou, Guan-Tarn Huang, Ki Hean Kim, Peter So, Yang-Fang Chen, Hsuan-Shu Lee, Chen-Yuan Dong

JBO, Vol. 15, Issue 03, 036002, (May 2010) https://doi.org/10.1117/12.10.1117/1.3427146

KEYWORDS: Liver, Second-harmonic generation, Collagen, Tissues, Multiphoton microscopy, Objectives, Physics, In vivo imaging, Imaging systems, Microscopy

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Proceedings Article | 26 February 2010 Paper

High-throughput three-dimensional (3D) lithographic microfabrication in biomedical applications

Daekeun Kim, Peter T. So

Proceedings Volume 7569, 75691V (2010) https://doi.org/10.1117/12.843160

KEYWORDS: Microfabrication, Lithography, 3D microstructuring, Biomedical optics, Photomasks, Fabrication, Microfluidics, Glasses, Coating, Prototyping

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Proceedings Article | 26 February 2010 Paper

Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy

Wei-Liang Chen, Tsung-Hsian Li, Ping-Jung Su, Chen-Kuan Chou, Peter Tramyeon Fwu, Sung-Jan Lin, Daekeun Kim, Peter T. So, Chen-Yuan Dong

Proceedings Volume 7569, 75691P (2010) https://doi.org/10.1117/12.843015

KEYWORDS: Second-harmonic generation, Polarization, Harmonic generation, Microscopy, Biomedical optics, Molecules, Microscopes, Mirrors, Fiber lasers, Tissues

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Proceedings Article | 23 February 2010 Paper

Human hepatocellular carcinoma diagnosis by multiphoton autofluorescence microscopy

Tzu-Lin Sun, Yuan Liu, Ming-Chin Sung, Hsiao-Ching Chen, Chuen-Huei Yang, Vladimir Hovhannisyan, Ling-Ling Chiou, Wei-Chou Lin, Guan-Tarn Huang, Ki-Hean Kim, Peter So, Chih-Ju Lin, Hsuan-Shu Lee, Chen-Yuan Dong

Proceedings Volume 7555, 75551L (2010) https://doi.org/10.1117/12.843157

KEYWORDS: Tissues, Liver, Second-harmonic generation, Multiphoton microscopy, Quantitative analysis, Tumors, Diagnostics, Physics, Signal processing, Microscopy

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Proceedings Article | 12 February 2010 Paper

Differentiation of normal and cancerous lung tissues by multiphoton imaging

Chun-Chin Wang, Feng-Chieh Li, Ruei-Jr Wu, Vladimir Hovhannisyan, Wei-Chou Lin, Sung-Jan Lin, Peter T. So, Chen-Yuan Dong

Proceedings Volume 7561, 756112 (2010) https://doi.org/10.1117/12.843173

KEYWORDS: Tissues, Lung, Second-harmonic generation, Lung cancer, Multiphoton microscopy, Cancer, Tumors, Collagen, Luminescence, Optical microscopy

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SPIE Journal Paper | 1 September 2009 Open Access

Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging

Wei-Liang Chen, Chen-Kuan Chou, Ming-Gu Lin, Yang-Fang Chen, Shiou-Hwa Jee, Hsian-Yuan Tan, Tsung-Hua Tsai, Ki Hean Kim, Daekeun Kim, Peter So, Sung-Jan Lin, Chen-Yuan Dong

JBO, Vol. 14, Issue 05, 054026, (September 2009) https://doi.org/10.1117/12.10.1117/1.3247157

KEYWORDS: Skin, Second-harmonic generation, Cornea, Signal detection, Confocal microscopy, Multiphoton microscopy, Tissues, Tissue optics, Refractive index, Roads

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SPIE Journal Paper | 1 July 2009 Open Access

Differentiation of normal and cancerous lung tissues by multiphoton imaging

Chun-Chin Wang, Feng-Chieh Li, Ruei-Jr Wu, Vladimir Hovhannisyan, Wei-Chou Lin, Sung-Jan Lin, Peter So, Chen-Yuan Dong

JBO, Vol. 14, Issue 04, 044034, (July 2009) https://doi.org/10.1117/12.10.1117/1.3210768

KEYWORDS: Tissues, Lung, Second-harmonic generation, Lung cancer, Cancer, Optical fibers, Multiphoton microscopy, Tumors, Collagen, Roads

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SPIE Journal Paper | 1 July 2009 Open Access

Three-dimensional cardiac architecture determined by two-photon microtomy

Hayden Huang, Catherine MacGillivray, Hyuk-Sang Kwon, Jan Lammerding, Jeffrey Robbins, Richard Lee, Peter So

JBO, Vol. 14, Issue 04, 044029, (July 2009) https://doi.org/10.1117/12.10.1117/1.3200939

KEYWORDS: Heart, Video, Tissues, 3D image processing, Two photon excitation microscopy, Mirrors, Quantitative analysis, Data acquisition, Imaging systems, Medicine

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SPIE Journal Paper | 1 July 2009 Open Access

Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy

Dean Tai, Nancy Tan, Shuoyu Xu, Chiang Huen Kang, Ser Mien Chia, Chee Leong Cheng, Aileen Wee, Chiang Li Wei, Anju Raja, Guangfa Xiao, Shi Chang, Jagath Rajapakse, Peter So, Hui-Huan Tang, Chien Shing Chen, Hanary Yu

JBO, Vol. 14, Issue 04, 044013, (July 2009) https://doi.org/10.1117/12.10.1117/1.3183811

KEYWORDS: Liver, Collagen, Second-harmonic generation, Tissues, Microscopy, Image segmentation, Algorithm development, Tissue optics, Harmonic generation, Animal model studies

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Proceedings Article | 13 February 2009 Paper

Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation

Daekeun Kim, Peter T. So

Proceedings Volume 7183, 71831B (2009) https://doi.org/10.1117/12.810910

KEYWORDS: Optical resolution, 3D image processing, Objectives, Biomedical optics, Imaging systems, Diffraction gratings, Ultrafast phenomena, Integrated optics, Microscopy, Diffraction

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SPIE Journal Paper | 1 January 2009 Open Access

Book Review: Handbook of Biomedical Nonlinear Optical Microscopy

William Mantulin, Barry Masters, Peter So

JBO, Vol. 14, Issue 01, 019901, (January 2009) https://doi.org/10.1117/12.10.1117/1.3077566

KEYWORDS: Microscopy, Biomedical optics, Luminescence, Optical microscopy, Harmonic generation, Optical spectroscopy, Multiphoton microscopy, Tissues, Spectroscopy, Spectroscopes

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SPIE Journal Paper | 1 November 2008 Open Access

Multiscale structural analysis of mouse lingual myoarchitecture employing diffusion spectrum magnetic resonance imaging and multiphoton microscopy

Terry Gaige, Hyuk-Sang Kwon, Guangping Dai, Victor Cabral, Ruopeng Wang, Yoon Sung Nam, Bevin Engelward, Van Wedeen, Peter So, Richard Gilbert

JBO, Vol. 13, Issue 06, 064005, (November 2008) https://doi.org/10.1117/12.10.1117/1.3046724

KEYWORDS: Diffusion, Tissues, Structured optical fibers, Tongue, Magnetic resonance imaging, Structural analysis, 3D image processing, Objectives, Microscopes, Tissue optics

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Proceedings Article | 15 February 2008 Paper

Axial resolution for two-photon wide-field illumination microscopy and microfabrication

Daekeun Kim, Peter So

Proceedings Volume 6860, 686028 (2008) https://doi.org/10.1117/12.784748

KEYWORDS: Microscopy, Microfabrication, Multiphoton microscopy, Mathematical modeling, Optical resolution, Geometrical optics, Photopolymerization, Imaging systems, Objectives, Mirrors

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Proceedings Article | 14 February 2007 Paper

Toward the standing wave surface plasmon resonance fluorescence microscopy

Euiheon Chung, Wai Teng Tang, Yang-Hyo Kim, Colin J. R. Sheppard, Peter T. C. So

Proceedings Volume 6450, 64500A (2007) https://doi.org/10.1117/12.705720

KEYWORDS: Surface plasmons, Point spread functions, Microscopy, Luminescence, Metals, Resonance enhancement, Glasses, Microscopes, Objectives, Reflection

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Proceedings Article | 10 February 2007 Paper

Second-harmonic generation investigation of collagen thermal denaturation

Wei-Liang Chen, Yen Sun, Sung-Jan Lin, Shiou-Hwa Jee, Yang-Fang Chen, Ling-Chih Lin, Peter So, Chen-Yuan Dong

Proceedings Volume 6442, 64421D (2007) https://doi.org/10.1117/12.698524

KEYWORDS: Second-harmonic generation, Collagen, Microscopy, Mirrors, Optical fibers, Imaging systems, Physics, Medicine, Image resolution, Objectives

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SPIE Journal Paper | 1 January 2007 Open Access

High-resolution whole organ imaging using two-photon tissue cytometry

Timothy Ragan, Jeremy Sylvan, Ki Kim, Hayden Huang, Karsten Bahlmann, Richard Lee, Peter So

JBO, Vol. 12, Issue 01, 014015, (January 2007) https://doi.org/10.1117/12.10.1117/1.2435626

KEYWORDS: Tissues, Heart, 3D image processing, Image resolution, Tissue optics, Biology, Microscopes, Image segmentation, Stereoscopy, Imaging systems

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SPIE Journal Paper | 1 May 2006 Open Access

Comparing the quantification of Förster resonance energy transfer measurement accuracies based on intensity, spectral, and lifetime imaging

Serge Pelet, Michael Previte, Peter So

JBO, Vol. 11, Issue 03, 034017, (May 2006) https://doi.org/10.1117/12.10.1117/1.2203664

KEYWORDS: Fluorescence resonance energy transfer, Capillaries, Luminescence, Fluorescence lifetime imaging, Imaging spectroscopy, Photons, Microscopes, Proteins, Energy transfer, Quantum efficiency

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Proceedings Article | 30 March 2005 Paper

Optical biopsy in high-speed handheld miniaturized multifocal multiphoton microscopy

Daekeun Kim, Ki Hean Kim, Siavash Yazdanfar, Peter So

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.597055

KEYWORDS: Biopsy, Tissue optics, Skin, Photonic crystal fibers, Multiphoton microscopy, Mirrors, Tissues, Distortion, Microscopes, Dispersion

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SPIE Journal Paper | 1 March 2005 Open Access

Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra

Lily Hsu, Serge Pelet, Thomas Hancewicz, Peter Kaplan, Peter So

JBO, Vol. 10, Issue 02, 024016, (March 2005) https://doi.org/10.1117/12.10.1117/1.1891370

KEYWORDS: Skin, Luminescence, Tissues, Tissue optics, Spectroscopy, Photons, Collagen, Image filtering, 3D image processing, Second-harmonic generation

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SPIE Journal Paper | 1 November 2004 Open Access

Mitigating thermal mechanical damage potential during two-photon dermal imaging

Barry Masters, Peter So, Christof Buehler, Nicholas Barry, Jason Sutin, William Mantulin, Enrico Gratton

JBO, Vol. 9, Issue 06, (November 2004) https://doi.org/10.1117/12.10.1117/1.1806135

KEYWORDS: Pulsed laser operation, Skin, Two photon imaging, Absorption, Laser energy, Cavitation, Luminescence, Laser damage threshold, In vivo imaging, Infrared radiation

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Proceedings Article | 21 June 2004 Paper

Signal degradation in multiple-scattering medium: implication for single focus vs multifoci two-photon microscopy

Ki Kim, Christof Buehler, Karsten Bahlmann, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.532396

KEYWORDS: Charge-coupled devices, Point spread functions, Scattering, Sensors, Signal detection, Light scattering, Two photon excitation microscopy, Neurons, Brain, CCD image sensors

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Proceedings Article | 21 June 2004 Paper

Development of an improved FRET-imaging two-photon microscope combining lifetime and spectral imaging

Serge Pelet, Micheal Previte, Ki Hean Kim, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.537620

KEYWORDS: Fluorescence resonance energy transfer, Modulation, Luminescence, Imaging spectroscopy, Phase shift keying, Microscopes, Fluorescence lifetime imaging, Proteins, Algorithm development, Phase measurement

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Proceedings Article | 21 June 2004 Paper

High-speed handheld multiphoton multifoci microscopy

Daekeun Kim, Ki Kim, Siavash Yazdanfar, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.530395

KEYWORDS: Multiphoton microscopy, Mirrors, Microscopy, Optical fibers, Imaging systems, Beam splitters, Luminescence, High speed imaging, Biopsy, Tissue optics

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SPIE Journal Paper | 1 July 2003 Open Access

Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium

Chen-Yuan Dong, Karsten Koenig, Peter So

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1578644

KEYWORDS: Point spread functions, Scattering, Objectives, Luminescence, Two photon excitation microscopy, Water, Data modeling, Tissues, Optical spheres, Photons

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Proceedings Article | 17 June 2002 Paper

Characterization of two-photon point-spread-function in skin imaging applications

Chen-Yuan Dong, Betty Yu, Lily Hsu, Peter So

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470680

KEYWORDS: Skin, Point spread functions, Water, Two photon excitation microscopy, Luminescence, Scattering, Objectives, Refractive index, Optical properties, Two photon imaging

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Proceedings Article | 17 June 2002 Paper

Two-photon 3D mapping of tisssue endogenous fluorescence species based on fluorescence emission spectra

Lily Hsu, Thomas Hancewicz, Peter Kaplan, Peter So

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470702

KEYWORDS: Skin, Luminescence, Tissues, Image analysis, Spectroscopy, Tissue optics, Image filtering, 3D image processing, Imaging spectroscopy, Collagen

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Proceedings Article | 17 June 2002 Paper

Multicolor two-photon scanning microscopy using a 16-channel photomultiplier

Christof Buehler, Ki Kim, Urs Greuter, Nick Schlumpf, Peter So

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470694

KEYWORDS: Luminescence, Microscopes, Photon counting, Imaging spectroscopy, Tissue optics, Field programmable gate arrays, Microscopy, Spectrographs, Photomultipliers, Spectral resolution

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Proceedings Article | 10 May 2001 Paper

Studying cellular mechanics using magnetic tweezers and two-photon microscopy

Hayden Huang, Chen-Yuan Dong, Hyuk-Sang Kwon, Jason Sutin, Roger Kamm, Peter So

Proceedings Volume 4260, (2001) https://doi.org/10.1117/12.426763

KEYWORDS: Magnetism, Cell mechanics, Two photon excitation microscopy, Transducers, Particles, Stereoscopy, Objectives, Image resolution, 3D image processing, Ferromagnetics

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Proceedings Article | 10 May 2001 Paper

Ultrahigh-resolution imaging of biological systems based on standing-wave total internal reflection microscopy

Peter So

Proceedings Volume 4260, (2001) https://doi.org/10.1117/12.426783

KEYWORDS: Point spread functions, Image resolution, Microscopy, Composites, Imaging systems, Optical microscopy, Resolution enhancement technologies, Algorithm development, Luminescence, Optical resolution

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Proceedings Article | 24 April 2001 Paper

Three-dimensional image cytometer based on a high-speed two-photon scanning microscope

Ki Kim, Molly Stitt, Carrie Hendricks, Karen Almeida, Bevin Engelward, Peter So

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424559

KEYWORDS: 3D image processing, Tissues, Mirrors, Microscopes, Objectives, Skin, Scanners, Fluorescent proteins, Microscopy, Polygon scanners

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We developed a 3-D image cytometer based on two-photon scanning microscopy. The system keeps the inherent advantages from two-photon scanning microscopy: (1) The ability of imaging thick tissue samples up to a few hundred micrometers, (2) The ability to study tissue structures with subcellular resolution, (3) The ability to monitor tissue biochemistry and metabolism, and (4) The reduction of specimen photobleaching and photodamage. Therefore, 3-D image cytometer has the ability to characterize multiple cell layer specimens, in contrast with 2-D image cytometer where only single cell layer samples can be imaged. 3-D image cytometry increases its frame rate by adapting a polygonal mirror scanner and high-speed photomultiplier tubes. The current frame rate is 13 frames per second. High throughput rate is achieved by imaging multiple cell layer specimens in 3-D at a high frame rate. The throughput rate of this system is dependent on the choice of objective lenses, specimen properties, and the speed of computer-controlled specimen stage. It can be up to approximately 100 cells per second which is comparable with that of 2-D image cytometers. With the high throughput rate and deep tissue imaging capability, 3-D image cytometer has the potential for the detection of rare cellular events inside living, intact tissues. A promising application of this 3-D image cytometer is the study of mitotic recombination in tissues. Mitotic recombination is a mechanism for genetic change. Therefore it is one of causes for carcinogenesis. However, the study of this process is difficult because recombination event is rare and it occurs at a rate of one cell in 10⁵ cells. The new method for the study is (1) to engineer transgenic mice whose cells will express fluorescence in the presence of mitotic recombination, (2) to detect cells which have undergone mitotic recombination with 3-D image cytometry. The estimated time required to quantify spontaneous recombination rate is approximately within a few hours in the case that the mutation occurs at a rate of 1/10⁵. The ability of this 3-D image cytometer to resolve tissue structures at video rate was demonstrated in the study of ex vivo human skin dermal structure. 25 X 25 section images were taken by shifting the acquisition region with computer- controlled specimen stage. Wide area images were reconstructed by combining each image sections. The size of complete wide area images is approximately 25 mm X 25 mm. We further performed experiments to verify this cytometer's ability for population statistics measurement. We prepared cell cultures containing a mixture of cells expressing cyan and yellow fluorescent proteins. These cell cultures with mixing ratio ranging from 1/10 up to 1/10⁵ were imaged. Experimental results show that the presence of a few rare cells in large pool of the other cells can be quantitatively measured. We also imaged a punched ear specimen from a transgenic mouse which carries green fluorescent protein. The data demonstrates that this system can resolve a single green fluorescent cells in the tissue.

Proceedings Article | 24 April 2001 Paper

Application of two-photon microscopy to elucidate oleic-acid-induced changes in microscale transdermal transport processes

Betty Yu, Chen-Yuan Dong, Peter So, Daniel Blankschtein, Robert Langer

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424557

KEYWORDS: Skin, Diffusion, Luminescence, Visualization, 3D scanning, Microscopes, Photons, Molecules, Two photon excitation microscopy, Microscopy

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Proceedings Article | 24 April 2001 Paper

Two-photon 3D mapping of tissue endogenous fluorescence species based on fluoresence excitation spectra

Lily Hsu, Thomas Hancewicz, Peter Kaplan, Keith Berland, Peter So

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424567

KEYWORDS: Skin, Tissues, Luminescence, Spectroscopy, Chemical analysis, Tissue optics, Photons, 3D image processing, Physiology, Image resolution

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Proceedings Article | 24 April 2001 Paper

Two-photon confocal microscopy in wound healing

Fernando Navarro, Peter So, Antoine Driessen, Nina Kropf, Christine Park, Juan Huertas, Hoon Lee, Dennis Orgill

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424579

KEYWORDS: Skin, Collagen, Microscopy, Microscopes, Two photon confocal microscopy, Wound healing, Tissues, Laser scattering, 3D image processing, Objectives

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Proceedings Article | 24 April 2001 Paper

3D particle tracking on the two-photon microscope

Timothy Ragan, Peter So, Hyuk-Sang Kwon, Enrico Gratton

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424560

KEYWORDS: Particles, Diffusion, Microscopes, Optical spheres, Point spread functions, Motion models, 3D modeling, Systems modeling, Microscopy, Objectives

Proceedings Article | 24 April 2001 Paper

Characterization of two-photon point-spread function in turbid medium by direct measurements, multicolor imaging, and blind deconvolution

Chen-Yuan Dong, Eric Bevan, Lily Hsu, Karsten Koenig, Peter So

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424538

KEYWORDS: Point spread functions, Scattering, Tissues, Two photon excitation microscopy, Deconvolution, Luminescence, Biomedical optics, Objectives, Microscopes, Optical spheres

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Proceedings Article | 4 July 2000 Paper

Applications of two-photon fluorescence microscopy in deep-tissue imaging

Chen-Yuan Dong, Betty Yu, Lily Hsu, Peter Kaplan, D. Blankschstein, Robert Langer, Peter So

Proceedings Volume 4082, (2000) https://doi.org/10.1117/12.390532

KEYWORDS: Skin, Luminescence, Two photon excitation microscopy, Photons, Molecules, Microscopes, Deconvolution, Objectives, Absorption, Point spread functions

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Proceedings Article | 27 April 2000 Paper

Magnetic tweezers microscope for cellular manipulation

Chen-Yuan Dong, Hayden Huang, Jason Sutin, Hyuk-Sang Kwon, George Cragg, R. Gilbert, Richard Lee, Enrico Gratton, Roger Kamm, Douglas Lauffenburger, Peter So

Proceedings Volume 3921, (2000) https://doi.org/10.1117/12.384210

KEYWORDS: Magnetism, Particles, Microscopes, Optical spheres, Calibration, Finite element methods, Magnetic tracking, Data acquisition, Iron, Image resolution

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Proceedings Article | 27 April 2000 Paper

Two-photon fluorescence confocal microscopy with a passively Q-switched Nd:YAG microchip laser

Colin Brenan, Peter So

Proceedings Volume 3921, (2000) https://doi.org/10.1117/12.384222

KEYWORDS: Luminescence, Absorption, Pulsed laser operation, Microscopes, Confocal microscopy, Two photon excitation microscopy, Q switched lasers, Objectives, Nd:YAG lasers, Semiconductor lasers

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Proceedings Article | 6 April 2000 Paper

Multiphoton excitation microscopy of human skin in vivo: early development of an optical biopsy

Barry Masters, Peter So

Proceedings Volume 4001, (2000) https://doi.org/10.1117/12.381485

KEYWORDS: Skin, Confocal microscopy, Microscopes, Tissue optics, Microscopy, In vivo imaging, Multiphoton microscopy, Objectives, Luminescence, Biopsy

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Proceedings Article | 1 June 1999 Paper

Tissue imaging using two-photon video rate microscopy

Ki Kim, Christof Buehler, Chen-Yuan Dong, Barry Masters, Peter So

Proceedings Volume 3604, (1999) https://doi.org/10.1117/12.349216

KEYWORDS: Tissue optics, Microscopes, Luminescence, Tissues, Confocal microscopy, Mirrors, Skin, Two photon imaging, Video, CCD cameras

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Proceedings Article | 6 May 1999 Open Access

Spatial point spread function with depth in two-photon microscopy

Andrew Dunn, Vincent Wallace, Mariah Coleno, Peter So, Bruce Tromberg

Proceedings Volume 3605, (1999) https://doi.org/10.1117/12.347559

KEYWORDS: Luminescence, Scattering, Monte Carlo methods, Point spread functions, Optical spheres, Optical properties, Signal detection, Two photon imaging, Signal attenuation, Tissue optics

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Proceedings Article | 21 April 1999 Paper

Frequency-domain pump-probe microscopic imaging using intensity-modulated laser diodes

Christof Buehler, Chen-Yuan Dong, Peter So, Todd French, Enrico Gratton

Proceedings Volume 3603, (1999) https://doi.org/10.1117/12.346749

KEYWORDS: Semiconductor lasers, Luminescence, Modulation, Microscopy, Laser beam diagnostics, Pulsed laser operation, Signal detection, Spatial resolution, Optical spheres, Microscopes

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Proceedings Article | 29 April 1998 Paper

Two-photon fluorescence and confocal reflected light imaging of thick tissue structures

Ki Kim, Peter So, Irene Kochevar, Barry Masters, Enrico Gratton

Proceedings Volume 3260, (1998) https://doi.org/10.1117/12.307081

KEYWORDS: Confocal microscopy, Luminescence, Tissues, Tissue optics, Microscopes, Skin, Photons, Chromophores, Objectives, Optical spheres

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Proceedings Article | 23 May 1997 Paper

Two-photon single particle tracking in 3D

Peter So, Timothy Ragan, Enrico Gratton, Jenny Carerro, Edward Voss

Proceedings Volume 2983, (1997) https://doi.org/10.1117/12.274323

KEYWORDS: Particles, Optical spheres, Diffusion, Latex, Luminescence, Photons, Proteins, Confocal microscopy, Microscopy, Receptors

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Proceedings Article | 23 May 1997 Open Access

Cell damage in UVA and cw/femtosecond NIR microscopes

Karsten Koenig, Hong Liang, Sol Kimel, Lars Svaasand, Bruce Tromberg, Tatiana Krasieva, Michael Berns, Karl-Juergen Halbhuber, Peter So, William Mantulin, Enrico Gratton

Proceedings Volume 2983, (1997) https://doi.org/10.1117/12.274337

KEYWORDS: Near infrared, Femtosecond phenomena, Microscopes, Continuous wave operation, Luminescence, Objectives, Absorption, Optical tweezers, Microscopy, Cell death

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Proceedings Article | 6 December 1996 Paper

Cell damage in two-photon microscopes

Karsten Koenig, Peter So, William Mantulin, Enrico Gratton

Proceedings Volume 2926, (1996) https://doi.org/10.1117/12.260794

KEYWORDS: Near infrared, Femtosecond phenomena, Luminescence, Microscopes, Photons, Objectives, Microscopy, Nondestructive evaluation, Plasma, 3D image processing

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Proceedings Article | 10 May 1996 Paper

Fluorescence lifetime microscopy: a stimulated emission approach

Enrico Gratton, Chen Dong, Peter So

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239514

KEYWORDS: Microscopy, Luminescence, Microscopes, Laser beam diagnostics, Confocal microscopy, Spectroscopy, Modulation, Spatial resolution, Photons, Signal detection

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Proceedings Article | 15 January 1996 Open Access

Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microradiation

Karsten Koenig, Peter So, William Mantulin, Enrico Gratton, Tatiana Krasieva, Michael Berns, Bruce Tromberg

Proceedings Volume 2628, (1996) https://doi.org/10.1117/12.229988

KEYWORDS: Luminescence, Near infrared, Continuous wave operation, Laser scanners, Absorption, Femtosecond phenomena, Microscopes, Optical tweezers, Auto-fluorescence imaging

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Proceedings Article | 17 August 1994 Paper

Frequency domain time-resolved microscope using a fast-scan CCD camera

Peter So, Todd French, Enrico Gratton

Proceedings Volume 2137, (1994) https://doi.org/10.1117/12.182713

KEYWORDS: Modulation, Luminescence, Microscopes, Phase shift keying, Microchannel plates, Optical spheres, CCD cameras, Heterodyning, Cameras, Phase shifts

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Proceedings Article | 14 September 1993 Paper

Quantitative diffusive wave spectroscopy in tissues

William Mantulin, Joshua Fishkin, Peter So, Enrico Gratton, John Maier

Proceedings Volume 1888, (1993) https://doi.org/10.1117/12.154661

KEYWORDS: Absorption, Scattering, Modulation, Tissues, Photons, Phase shift keying, Scattering media, Light scattering, Spectroscopy, Diffusion

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Showing 5 of 115 publications

Proceedings Volume Editor (28)

SPIE Conference Volume | 4 April 2024

Multiphoton Microscopy in the Biomedical Sciences XXIV

SPIE Conference Volume | 25 April 2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

SPIE Conference Volume | 11 April 2022

Multiphoton Microscopy in the Biomedical Sciences XXII

SPIE Conference Volume | 16 April 2021

Multiphoton Microscopy in the Biomedical Sciences XXI

SPIE Conference Volume | 5 March 2020

Multiphoton Microscopy in the Biomedical Sciences XX

Showing 5 of 28 publications

Conference Committee Involvement (78)

Multiphoton Microscopy in the Biomedical Sciences XXV

26 January 2025 | San Francisco, California, United States

High-Throughput Biophotonics: Imaging, Spectroscopy, and Beyond X

25 January 2025 | San Francisco, California, United States

Optical Elastography and Tissue Biomechanics XII

25 January 2025 | San Francisco, California, United States

Quantitative Phase Imaging XI

25 January 2025 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXIV

28 January 2024 | San Francisco, California, United States

Quantitative Phase Imaging X

27 January 2024 | San Francisco, California, United States

Optical Elastography and Tissue Biomechanics XI

27 January 2024 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy IX

27 January 2024 | San Francisco, California, United States

European Conferences on Biomedical Optics

25 June 2023 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XXIII

29 January 2023 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy VIII

28 January 2023 | San Francisco, California, United States

Quantitative Phase Imaging IX

28 January 2023 | San Francisco, California, United States

Optical Elastography and Tissue Biomechanics X

28 January 2023 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXII

23 January 2022 | San Francisco, California, United States

Quantitative Phase Imaging VIII

22 January 2022 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy VII

22 January 2022 | San Francisco, California, United States

Optical Elastography and Tissue Biomechanics IX

22 January 2022 | San Francisco, California, United States

Optical Elastography and Tissue Biomechanics VIII

6 March 2021 | Online Only, California, United States

High-Speed Biomedical Imaging and Spectroscopy VI

6 March 2021 | Online Only, California, United States

Multiphoton Microscopy in the Biomedical Sciences XXI

6 March 2021 | Online Only, California, United States

Quantitative Phase Imaging VII

6 March 2021 | Online Only, California, United States

Multiphoton Microscopy in the Biomedical Sciences XX

2 February 2020 | San Francisco, California, United States

Quantitative Phase Imaging VI

1 February 2020 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy V

1 February 2020 | San Francisco, California, United States

Advances in Microscopic Imaging

26 June 2019 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XIX

3 February 2019 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy IV

2 February 2019 | San Francisco, California, United States

Quantitative Phase Imaging V

2 February 2019 | San Francisco, California, United States

Endoscopic Microscopy XIV

2 February 2019 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management

29 January 2018 | San Francisco, California, United States

Quantitative Phase Imaging IV

28 January 2018 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVIII

28 January 2018 | San Francisco, California, United States

Endoscopic Microscopy XIII

27 January 2018 | San Francisco, California, United States

Advances in Microscopic Imaging

25 June 2017 | Munich, Germany

High-Speed Biomedical Imaging and Spectroscopy II: Toward Big Data Instrumentation and Management

30 January 2017 | San Francisco, California, United States

Quantitative Phase Imaging III

29 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVII

29 January 2017 | San Francisco, California, United States

Endoscopic Microscopy XII

29 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVI

14 February 2016 | San Francisco, California, United States

Quantitative Phase Imaging II

14 February 2016 | San Francisco, California, United States

Endoscopic Microscopy XI

14 February 2016 | San Francisco, California, United States

High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management

13 February 2016 | San Francisco, California, United States

Advanced Microscopy Techniques IV

24 June 2015 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XV

8 February 2015 | San Francisco, California, United States

Endoscopic Microscopy X

8 February 2015 | San Francisco, California, United States

Quantitative Phase Imaging

7 February 2015 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIV

2 February 2014 | San Francisco, California, United States

Endoscopic Microscopy IX

2 February 2014 | San Francisco, California, United States

Advanced Microscopy Techniques III

12 May 2013 | Munich, Germany

Endoscopic Microscopy VIII

3 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIII

3 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XII

22 January 2012 | San Francisco, California, United States

Endoscopic Microscopy VII

22 January 2012 | San Francisco, California, United States

Advanced Microscopy Techniques II

22 May 2011 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XI

23 January 2011 | San Francisco, California, United States

Endoscopic Microscopy VI

23 January 2011 | San Francisco, California, United States

Endoscopic Microscopy V

25 January 2010 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences X

24 January 2010 | San Francisco, California, United States

Advanced Photon Counting Techniques III

14 April 2009 | Orlando, Florida, United States

Multiphoton Microscopy in the Biomedical Sciences IX

25 January 2009 | San Jose, California, United States

Endoscopic Microscopy IV

25 January 2009 | San Jose, California, United States

Endoscopic Microscopy III

20 January 2008 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences VIII

20 January 2008 | San Jose, California, United States

Advanced Photon Counting Techniques II

9 September 2007 | Boston, MA, United States

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

17 June 2007 | Munich, Germany

Endoscopic Microscopy II

21 January 2007 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences VII

21 January 2007 | San Jose, California, United States

Advanced Photon Counting Techniques

1 October 2006 | Boston, Massachusetts, United States

Multiphoton Microscopy in the Biomedical Sciences VI

22 January 2006 | San Jose, California, United States

Endoscopic Microscopy

22 January 2006 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences V

23 January 2005 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences IV

25 January 2004 | San Jose, CA, United States

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

22 June 2003 | Munich, Germany

Manipulation and Analysis of Biomolecules, Cells and Tissues

28 January 2003 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences III

26 January 2003 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences II

20 January 2002 | San Jose, CA, United States

Multiphoton Microscopy in the Biomedical Sciences

21 January 2001 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells and Tissues

24 January 2000 | San Jose, CA, United States

Showing 5 of 78 Conference Committees

Course Instructor

SC819: Multiphoton Microscopy (MM) - Basics, Technology Development, and Applications

This course introduces the basics of multiphoton microscopy, technology development, image acquisition and analysis related to various biological and clinical applications. The first part of the course will cover the basics of microscopy, image formation, detectors, lenses used in MM, and calculation of absorption cross-sections. The second part of the course will focus on how to build your own MM, or integrate one with an existing confocal microscope. The tricks involved in MM development and imaging will be covered. Finally, we will review many applications including tissue imaging, MM in Neuro and developmental biology, labeling issues for MM imaging, FLIM, FRET and other clinical applications.

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