Dr. Shih-Mo Yang Profile

Dr. Shih-Mo Yang

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Proceedings Article | 28 August 2010 Paper

A novel optoelectrofulidic system for cells/particles manipulation and sorting

Shih-Mo Yang, Tung-Ming Yu, Hang-Ping Huang, Long Hsu, Cheng-Hsien Liu

Proceedings Volume 7762, 77621W (2010) https://doi.org/10.1117/12.860599

KEYWORDS: Particles, Dielectrophoresis, Interfaces, Electrodes, Switches, Glasses, Optoelectronics, Liquids, Control systems, Optical tweezers

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A novel optoelectrofulidic system integrated optical image concentration and alignment system, dielectrophoresis phenomenon, microfluidic and friendly real-time control interface is first reported in this article. A new application of photoconductive material oxotitanium phthalocyanine (TiOPc) for microparticle applying has been first described and demonstrated by our research group. Basis on the special character of the photoconductive material, a TiOPc-based optoelectronic tweezers (Ti-OET) is utilized for single and massive cells/particles manipulation. The objects wanted to be manipulated are defined with different behaviors (e.g., press, release, drag and move) using Flash® software when the cursor acts on them. It also reveals the application for biological application to form the cells trapping with three sorts of cells, HMEC-1, HepG2 and HEK293t. Another application of our optoelectrofulidic system is to fabricate a TiOPc-based flow cytometry chip which can be used for sorting the 15μm diameter particles with 105 μm/s velocity. When the 10Vp.p. voltage and 45 kHz AC frequency apply on the top and button ITO electrode, the illuminated light pattern will become a spatially virtual switch inside the microchannel. The dielectrophoresis force between top ITO glass and button photoconductive layer controlled by the friendly interface will concentrate the cells/particles as a straight line and individually direct each one in different paths. In summary, we have established an optoelectronfulidic-based chip and spatially virtual switch system which are applied for cell pattern and particles sorting. In the future, this easy manipulation approach can place the full power of optoelectronfulidic chip into the biological operators' hands.

Proceedings Article | 28 August 2010 Paper

Measurement of macrophage adhesion using optical tweezers with backward-scattered detection

Sung-Yang Wei, Yi-Jr Su, Po-Chen Shih, Shih-Mo Yang, Long Hsu

Proceedings Volume 7762, 77622M (2010) https://doi.org/10.1117/12.860357

KEYWORDS: Optical tweezers, Adhesives, Particles, Geometrical optics, Beam splitters, Objectives, Tissues, Inflammation, Blood circulation, Capillaries

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Macrophages are members of the leukocyte family. Tissue damage causes inflammation and release of vasoactive and chemotactic factors, which trigger a local increase in blood flow and capillary permeability. Then, leukocytes accumulate quickly to the infection site. The leukocyte extravasation process takes place according to a sequence of events that involve tethering, activation by a chemoattractant stimulus, adhesion by integrin binding, and migrating to the infection site. The leukocyte extravasation process reveals that adhesion is an important part of the immune system. Optical tweezers have become a useful tool with broad applications in biology and physics. In force measurement, the trapped bead as a probe usually uses a polystyrene bead of 1 μm diameter to measure adhesive force between the trapped beads and cell by optical tweezers. In this paper, using the ray-optics model calculated trapping stiffness and defined the linear displacement ranges. By the theoretical values of stiffness and linear displacement ranges, this study attempted to obtain a proper trapped particle size in measuring adhesive force. Finally, this work investigates real-time adhesion force measurements between human macrophages and trapped beads coated with lipopolysaccharides using optical tweezers with backscattered detection.

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