Prof. Živilė Lukšienė Profile

Prof. Živilė Lukšienė

at Vilnius Univ

SPIE Involvement:

Author

Proceedings Article | 14 August 2019 Presentation

Chlorophyllin-based photosensitization for microbial control of fresh produce (Conference Presentation)

Zivile Luksiene

Proceedings Volume 11070, 110700M (2019) https://doi.org/10.1117/12.2525570

KEYWORDS: Pathogens, Biomedical optics, Oxygen, Photosensitizer targeting, Microorganisms, Thermal effects, Coating

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Despite tremendous progress in biomedical science, the number of reported food-borne diseases continues to rise. Health experts estimate that every year food-borne illnesses in USA cost 86 billion dollars. Obviously, existing antibacterial technologies for microbial control of foods are not enough effective. Photosensitization is a treatment involving the interaction of the two non-toxic factors, photosensitizer and light, which in the presence of oxygen results in the destruction of the target cell without leakage of harmful by-products in the environment. According to our results, chlorophyllin (food additive E140) exhibits perfect photosensitizing properties. After excitation with light (405 nm) it inactivates food pathogens, their spores and biofilms, yeasts/microfungi. ROS-induced oxidative stress and following membrane damage was the main reason of photosensitization- based inactivation of microorganisms. Afterwards we applied photosensitization for microbial control of fresh produce. Obtained results indicate that this treatment significantly (2-3 log CFU/g) reduces microbial load on fruits (strawberries, apricots, plums), vegetables (cauliflower, cucumber, lettuce, basil) and sprouts without thermal effects on food matrix. Moreover, this treatment extended the shelf-life of treated produce by 2-4 days what is economically very important. No reduction of nutritional value (antioxidant activity, chlorophyll content) or organoleptic properties (color, texture, taste) of treated produce has been observed. In order to decontaminate fresh produce from Gram (+) and Gram (-) pathogens in uniform way, chlorophyllin was conjugated with chitosan. Obtained data reveal that such photoactivated conjugate is very effective against all pathogens and can be applied for coating of fresh produce. Therefore, a photosensitization phenomenon might open a new avenue for the development of non-thermal, effective and ecologically friendly antimicrobial technology for preservation of fresh produce.

Proceedings Article | 10 September 2004 Paper

LED-based light source for photodynamic inactivation of leukemia cells in vitro

Zivile Luksiene, Juozas Astrauskas, Iyad Kabbara

Proceedings Volume 5610, (2004) https://doi.org/10.1117/12.584436

KEYWORDS: Light sources, Diodes, Light emitting diodes, Photodynamic therapy, Leukemia, In vitro testing, Cancer, Light, Lamps, Visible radiation

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Proceedings Article | 10 September 2004 Paper

Hypericin-based photodynamic therapy: antitumor activity, accumulation potential, and induced cell death pathway

Zivile Luksiene, Aurelija Vaitkuviene

Proceedings Volume 5610, (2004) https://doi.org/10.1117/12.584383

KEYWORDS: Tumors, Photodynamic therapy, Cell death, Luminescence, Proteins, Light sources, Light, In vitro testing, In vivo imaging, Tungsten

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Proceedings Article | 29 December 1997 Paper

Experimental study of simultaneous photodynamic and hyperthermic treatment of Ehrlich ascites carcinoma and A22 hepatoma

Zivile Luksiene

Proceedings Volume 3191, (1997) https://doi.org/10.1117/12.297825

KEYWORDS: Tumors, Photodynamic therapy, Blood vessels

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Proceedings Article | 4 December 1996 Paper

Enhancement of photosensitization efficiency by various combinations with radiosensitization in an experimental Ehrlich ascites tumor

Zivile Luksiene, G. Kaspariunaite, E. Aleknavicius, Konstantinas Valuckas

Proceedings Volume 2924, (1996) https://doi.org/10.1117/12.260776

KEYWORDS: Tumors, Photodynamic therapy, Visible radiation, Luminescence, Ionizing radiation, Absorbance, Oncology, Light, Clinical trials, Cancer

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Proceedings Article | 12 January 1995 Paper

Combination of photodynamic therapy and x-irradiation: a study on 5-aminolevulinic acid (ALA) radiomodifying properties

Zivile Luksiene, Kristian Berg, Johan Moan

Proceedings Volume 2325, (1995) https://doi.org/10.1117/12.199159

KEYWORDS: Photodynamic therapy, X-rays, Ionizing radiation, Cancer, Radiotherapy, Gamma radiation, Fluorescence correlation spectroscopy, OSLO, Tissues, In vivo imaging

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Proceedings Article | 1 March 1994 Paper

Radiosensitization by porphyrins in 60Co-irradiated cells of different natures

Zivile Luksiene, Vydmantas Atkocius

Proceedings Volume 2078, (1994) https://doi.org/10.1117/12.168687

KEYWORDS: Absorbance, Photodynamic therapy, Tissues, Radiotherapy, In vitro testing, In vivo imaging, Tumors, Cancer, Oncology, Copper

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Proceedings Article | 18 June 1993 Paper

Porphyrins as radiosensitizers in 60Co-irradiated E. ascites tumor cells: possibility to combine with PDT

Zivile Luksiene, B. Juodka

Proceedings Volume 1881, (1993) https://doi.org/10.1117/12.146296

KEYWORDS: Tumors, Photodynamic therapy, Tumor growth modeling, Biophysics, Radiotherapy, Human-computer interaction, Absorbance, Microscopes, In vitro testing, Oncology

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Proceedings Article | 18 June 1993 Paper

Laser, gamma irradiation, and chemotherapy in treatment of experimental tumors sensitized by porphyrins

Konstantinas Valuckas, Janina Didziapetriene, Vydmantas Atkocius, Laima Griciute, Zivile Luksiene, Laima Zaleckaite, Ricardas Rotomskis, Valerijus Smilgevicius

Proceedings Volume 1881, (1993) https://doi.org/10.1117/12.146297

KEYWORDS: Photodynamic therapy, Radiotherapy, Toxicity, Pulsed laser operation, Tumors, Tissues, Melanoma, Calcium, Oncology, Absorption spectroscopy

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Showing 5 of 9 publications

Conference Committee Involvement (1)

17th International Photodynamic Association World Congress

28 June 2019 | Cambridge, Massachusetts, United States

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