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CONTENTS

In vivo imaging of light-emitting probes

[+] Author Affiliations
B. W. Rice, M. D. Cable, M. B. Nelson

Xenogen Corporation, 860 Atlantic Avenue, Alameda, California?94501

J. Biomed. Opt. 6(4), 432-440 (Oct 01, 2001). doi:10.1117/1.1413210
History: Received Jan. 26, 2001; Revised July 16, 2001; Accepted July 17, 2001
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In vivo imaging of cells tagged with light-emitting probes, such as firefly luciferase or fluorescent proteins, is a powerful technology that enables a wide range of biological studies in small research animals. Reporters with emission in the red to infrared (>600 nm) are preferred due to the low absorption in tissue at these wavelengths. Modeling of photon diffusion through tissue indicates that bioluminescent cell counts as low as a few hundred can be detected subcutaneously, while 106 cells are required to detect signals at ∼2 cm depth in tissue. Signal-to-noise estimates show that cooled back-thinned integrating charge coupled devices (CCDs) are preferred to image-intensified CCDs for this application, mainly due to their high quantum efficiency (∼85%) at wavelengths >600 nm where tissue absorption is low. Instrumentation for in vivo imaging developed at Xenogen is described and several examples of images of mice with bioluminescent cells are presented. © 2001 Society of Photo-Optical Instrumentation Engineers.

© 2001 Society of Photo-Optical Instrumentation Engineers

Citation

B. W. Rice ; M. D. Cable and M. B. Nelson
"In vivo imaging of light-emitting probes", J. Biomed. Opt. 6(4), 432-440 (Oct 01, 2001). ; http://dx.doi.org/10.1117/1.1413210


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