Research Papers

In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe

[+] Author Affiliations
L. Chrit

L’Oréal Recherche, Aulanay sous Bois, France, and, Unité Médian CNRS UMR 6142, UFR Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

C. Hadjur

L’Oréal Recherche, Aulnay sous Bois, France

S. Morel

Horiba Jobin Yvon S.A.S., Division Raman, 231 rue de Lille, 59650 Villeneuve d’Ascq, France

G. Sockalingum

Unité Médian CNRS UMR 6142, UFR Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

G. Lebourdon

Horiba Jobin Yvon S.A.S., Division Raman, 231 rue de Lille, 59650 Villeneuve d’Ascq, France

F. Leroy

L’Oréal Recherche, Aulnay sous Bois, France

M. Manfait

Unité Médian CNRS UMR 6142, UFR Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

J. Biomed. Opt. 10(4), 044007 (August 30, 2005). doi:10.1117/1.2003747
History: Received January 10, 2005; Revised April 09, 2005; Accepted April 12, 2005; Published August 30, 2005
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To evaluate the potential of a new in vivo confocal Raman microprobe, we undertake a pilot study in human skin. A fiber optic probe is operated with a 633-nm laser and trials are conducted in healthy volunteers. We examine changes in molecular composition and structure of the stratum corneum, from different volunteers, from different anatomical sites and skin layers. Main spectral variations are detected in the following regions: 800to900cm1 (amino acids); 1200to1290cm1 (proteins); and 1030to1130cm1, 1300to1450cm1, and 2800to2900cm1 (lipids). Curve fitting of the amide 1 region performs in detail protein secondary structural variations of the amide 1 band. Protein conformation is also found to vary depending on the anatomical site and volunteer. Similar analysis of the 730-to1170-cm1 spectral window reveals a different organization of lamellar lipids: gel for forearm and palm, and liquid-crystalline phase for fingertips. All these variations result from changes in the stratum corneum components such as natural moisturizing factor (NMF), lipids (namely ceramides), and water. Hierarchical clustering classification is also performed to sort out Raman data obtained from different subjects. Further improvement of the confocal probe would be to adapt a 360-deg configuration enabling access to other anatomical sites.

Figures in this Article
© 2005 Society of Photo-Optical Instrumentation Engineers

Topics

Skin ; Water

Citation

L. Chrit ; C. Hadjur ; S. Morel ; G. Sockalingum ; G. Lebourdon, et al.
"In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe", J. Biomed. Opt. 10(4), 044007 (August 30, 2005). ; http://dx.doi.org/10.1117/1.2003747


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