Research Papers

Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug

[+] Author Affiliations
Vladimir P. Zharov, Valentin Galitovskiy

University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205-7199

Christopher S. Lyle, Timothy C. Chambers

University of Arkansas for Medical Sciences, Department of Biochemistry and Molecular Biology, Little Rock, Arkansas 72205-7199

J. Biomed. Opt. 11(6), 064034 (December 21, 2006). doi:10.1117/1.2405349
History: Received February 24, 2006; Revised June 21, 2006; Accepted July 13, 2006; Published December 21, 2006; Online December 21, 2006
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We describe and explore the capability of a photothermal (PT) assay with modified schematics for highly sensitive detection of individual cell response to antitumor drug impact in vitro. Specifically, we used the nonlinear differential PT test to measure distinctive changes of specific PT parameters after exposure of KB3 carcinoma cells to the antitumor drug vinblastine in the broad concentration range of 1010to300nM. Verification of the PT assay was performed by comparison with multidrug-resistant cells and comparison with conventional assays evaluating cell viability, cytochrome c release, apoptosis induction, and cell size. We demonstrate that this system is capable of detecting drug-induced signals at a concentration threshold sensitivity at least seven orders of magnitude better than existing assays. We anticipate that this technique may serve as a convenient and rapid analytical tool to evaluate the presence of intracellular drug, with applications in high throughput screening assays and for studying drug uptake and distribution in more complex biological or clinical samples.

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© 2006 Society of Photo-Optical Instrumentation Engineers

Citation

Vladimir P. Zharov ; Valentin Galitovskiy ; Christopher S. Lyle and Timothy C. Chambers
"Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug", J. Biomed. Opt. 11(6), 064034 (December 21, 2006). ; http://dx.doi.org/10.1117/1.2405349


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