Research Papers

In vitro model that approximates retinal damage threshold trends

[+] Author Affiliations
Michael L. Denton, Michael S. Foltz, Kurt J. Schuster, Gary D. Noojin

Northrop Grumman, Warfighter Concepts and Applications Department, San Antonio, Texas

Larry E. Estlack

Conceptual MindWorks, Inc., San Antonio, Texas

Robert J. Thomas

Air Force Research Laboratory, 711 HPW/RHDO, Brooks City-Base, Texas

J. Biomed. Opt. 13(5), 054014 (September 22, 2008). doi:10.1117/1.2981831
History: Received September 11, 2007; Revised March 21, 2008; Accepted April 28, 2008; Published September 22, 2008
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Without effective in vitro damage models, advances in our understanding of the physics and biology of laser-tissue interaction would be hampered due to cost and ethical limitations placed on the use of nonhuman primates. We extend our characterization of laser-induced cell death in an existing in vitro retinal model to include damage thresholds at 514 and 413nm. The new data, when combined with data previously reported for 532 and 458nm exposures, provide a sufficiently broad range of wavelengths and exposure durations (0.1to100s) to make comparisons with minimum visible lesion (in vivo) data in the literature. Based on similarities between in vivo and in vitro action spectra and temporal action profiles, the cell culture model is found to respond to laser irradiation in a fundamentally similar fashion as the retina of the rhesus animal model. We further show that this response depends on the amount of intracellular melanin pigmentation.

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© 2008 Society of Photo-Optical Instrumentation Engineers

Citation

Michael L. Denton ; Michael S. Foltz ; Kurt J. Schuster ; Gary D. Noojin ; Larry E. Estlack, et al.
"In vitro model that approximates retinal damage threshold trends", J. Biomed. Opt. 13(5), 054014 (September 22, 2008). ; http://dx.doi.org/10.1117/1.2981831


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