Corneal equivalents: a replacement model for in-vivo 1540-nm laser exposure studies

David J. Fletcher D.V.M.; Thomas E. Johnson; Michael A. Mitchell; Thomas E. Eurell; Pedro J. Rico; William P. Roach

doi:10.1117/12.386288

17 May 2000 Corneal equivalents: a replacement model for in-vivo 1540-nm laser exposure studies

David J. Fletcher D.V.M., Thomas E. Johnson, Michael A. Mitchell, Thomas E. Eurell, Pedro J. Rico, William P. Roach

Proceedings Volume 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X; (2000) https://doi.org/10.1117/12.386288
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We investigate the use of in-vitro corneal equivalents as a replacement model for in-vivo rabbit corneas used in laser damage threshold studies. In-vitro corneal equivalents (CE) were exposed to 0.8 millisecond (ms), 1540 nanometer (nm) single laser light pulses ranging from 124 J/cm² to 58 J/cm². After exposure, CE's were evaluated opthalmoscopcopically, imaged using confocal microscopy and examined histologically to investigate the mechanisms of laser induced damage. Preliminary results indicate that the 50% damage threshold for CE's, ED₅₀, is approximately 70 J/cm² with a 0.5 mm diameter spot size. Additional data points are required to determine a statistically significant ED₅₀. Preliminary exposures of in-vivo rabbit corneas using the same laser parameters as the CE's are also reported. Comparisons between the in-vivo and in-vitro models are presented. Histopathological images from both models show remarkable similarities in the location and extent of damage throughout the full thickness of each tissue.

Citation Download Citation

David J. Fletcher D.V.M., Thomas E. Johnson, Michael A. Mitchell, Thomas E. Eurell, Pedro J. Rico, and William P. Roach "Corneal equivalents: a replacement model for in-vivo 1540-nm laser exposure studies", Proc. SPIE 3907, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X, (17 May 2000); https://doi.org/10.1117/12.386288

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cornea

Cerium

In vivo imaging

Tissues

In vitro testing

Animal model studies

Confocal microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years