Research Papers

Classification of ultraviolet irradiated mouse skin histological stages by bimodal spectroscopy: multiple excitation autofluorescence and diffuse reflectance

[+] Author Affiliations
Marine Amouroux, Gilberto Díaz-Ayil, Walter C. P. M. Blondel

Nancy-University, Centre de Recherche en Automatique de Nancy, CRAN UMR 7039 CNRS, 2 avenue de la Forêt de Haye, 54516 Vandoeuvre-les-Nancy cedex, France

Geneviève Bourg-Heckly

Université Pierre et Marie Curie Paris 6, Laboratoire de Biophysique Moléculaire, Cellulaire et Tissulaire, BioMoCeTi UMR 7033 CNRS, Genopole Campus 1, 5 rue Henri Desbruères, 91030 Evry cedex, France

Agnès Leroux

Alexis Vautrin Cancer Center, Avenue de Bourgogne, 54511 Vandoeuvre-les-Nancy, cedex, France

François Guillemin

Nancy-University, Centre de Recherche en Automatique de Nancy and Alexis Vautrin Cancer Center, Avenue de Bourgogne, 54511 Vandoeuvre-les-Nancy, cedex, France

J. Biomed. Opt. 14(1), 014011 (February 12, 2009). doi:10.1117/1.3077194
History: Received May 13, 2008; Revised November 25, 2008; Accepted November 25, 2008; Published February 12, 2009
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Histopathological analysis and in vivo optical spectroscopy were used to discriminate several histological stages of UV-irradiated mouse skin. At different times throughout the 30-week irradiation, autofluorescence (AF) and diffuse reflectance (DR) spectra were acquired in a bimodal approach. Then skin was sampled and processed to be classified, according to morphological criteria, into four histological categories: normal, and three types of hyperplasia (compensatory, atypical, and dysplastic). After extracting spectral characteristics, principal component analysis (data reduction) and the k-nearest neighbor classifying method were applied to compare diagnostic performances of monoexcitation AF (based on each of the seven excitation wavelengths: 360, 368, 390, 400, 410, 420, and 430nm), multiexcitation AF (combining the seven excitation wavelengths), DR, and bimodal spectroscopies. Visible wavelengths are the most sensitive ones to discriminate compensatory from precancerous (atypical and dysplastic) states. Multiexcitation AF provides an average 6-percentage-point increased sensitivity compared to the best scores obtained with monoexcitation AF for all pairs of tissue categories. Bimodality results in a 4-percentage-point increase of specificity when discriminating the three types of hyperplasia. Thus, bimodal spectroscopy appears to be a promising tool to discriminate benign from precancerous stages; clinical investigations should be carried out to confirm these results.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Marine Amouroux ; Gilberto Díaz-Ayil ; Walter C. P. M. Blondel ; Geneviève Bourg-Heckly ; Agnès Leroux, et al.
"Classification of ultraviolet irradiated mouse skin histological stages by bimodal spectroscopy: multiple excitation autofluorescence and diffuse reflectance", J. Biomed. Opt. 14(1), 014011 (February 12, 2009). ; http://dx.doi.org/10.1117/1.3077194


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