Research Papers

Beyond the 1∕Tp limit: two-photon-excited fluorescence using pulses as short as sub-10-fs

[+] Author Affiliations
Shuo Pang, Alvin T. Yeh, Chao Wang, Kenith E. Meissner

Texas A&M University, Department of Biomedical Engineering, 337 Zachry Engineering Center, 3120 TAMU, College Station, Texas 77843

J. Biomed. Opt. 14(5), 054041 (October 30, 2009). doi:10.1117/1.3253388
History: Received April 29, 2009; Revised August 24, 2009; Accepted August 26, 2009; Published October 30, 2009
Text Size: A A A

Two-photon-excited fluorescence and second-harmonic generation are characterized as a function of laser pulse duration as short as sub-10-fs. A comparative study is performed where pulse duration is varied by introducing dispersion, as reported previously, and by tailoring pulse spectral width and minimizing its time-bandwidth product (transform-limited pulses). Experimental data and calculations show that characterizing a two-photon signal with the two schemes to vary pulse duration measures different phenomena. Two-photon signal characterization using dispersion-broadened pulses measures only the effect of chirp on the pulse two-photon-excitation spectrum and is independent of molecular response. Transform-limited pulses are used to measure the dependence of two-photon signal generation on pulse duration. Calculations show that deviation from the 1Tp relationship would be expected as the transform-limited pulse spectral width approaches the molecular two-photon absorption linewidth and exhibits asymptotic behavior for pulse spectral widths 10 times greater than the absorption linewidth. Experimental measurements are consistent with the predicted behavior. The impact of using ultrashort laser pulses on the performance characteristics of nonlinear optical microscopy is discussed.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers


Shuo Pang ; Alvin T. Yeh ; Chao Wang and Kenith E. Meissner
"Beyond the 1∕Tp limit: two-photon-excited fluorescence using pulses as short as sub-10-fs", J. Biomed. Opt. 14(5), 054041 (October 30, 2009). ; http://dx.doi.org/10.1117/1.3253388

Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content


Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles

Buy this article ($18 for members, $25 for non-members).
Sign In