Research Papers: Imaging

Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord

[+] Author Affiliations
Yunzhou Shi

Purdue University, Weldon School of Biomedical Engineering,West Lafayette, Indiana 47907

Delong Zhang, Terry B. Huff

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

Xiaofei Wang

Indiana University School of Medicine, Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute and Department of Neurological Surgery, Indianapolis, Indiana 46202

Riyi Shi

Purdue University, Weldon School of Biomedical Engineering,West Lafayette, Indiana 47907

Purdue University, Department of Basic Medical Sciences and Center for Paralysis Research, West Lafayette, Indiana 47907

Xiao-Ming Xu

Purdue University, Department of Basic Medical Sciences and Center for Paralysis Research, West Lafayette, Indiana 47907

Ji-Xin Cheng

Purdue University, Weldon School of Biomedical Engineering,West Lafayette, Indiana 47907

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

J. Biomed. Opt. 16(10), 106012 (October 03, 2011). doi:10.1117/1.3641988
History: Received July 11, 2011; Revised August 30, 2011; Accepted September 01, 2011; Published October 03, 2011; Online October 03, 2011
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In vivo imaging of white matter is important for the mechanistic understanding of demyelination and evaluation of remyelination therapies. Although white matter can be visualized by a strong coherent anti-Stokes Raman scattering (CARS) signal from axonal myelin, in vivo repetitive CARS imaging of the spinal cord remains a challenge due to complexities induced by the laminectomy surgery. We present a careful experimental design that enabled longitudinal CARS imaging of de- and remyelination at single axon level in live rats. In vivo CARS imaging of secretory phospholipase A2 induced myelin vesiculation, macrophage uptake of myelin debris, and spontaneous remyelination by Schwann cells are sequentially monitored over a 3 week period. Longitudinal visualization of de- and remyelination at a single axon level provides a novel platform for rational design of therapies aimed at promoting myelin plasticity and repair.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Yunzhou Shi ; Delong Zhang ; Terry B. Huff ; Xiaofei Wang ; Riyi Shi, et al.
"Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord", J. Biomed. Opt. 16(10), 106012 (October 03, 2011). ; http://dx.doi.org/10.1117/1.3641988


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