Special Section on Optical Diagnostic and Biophotonic Methods from Bench to Bedside

Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering

[+] Author Affiliations
Paulius Danilevicius, Sima Rekstyte, Roaldas Gadonas, Mangirdas Malinauskas

Vilnius University, Faculty of Physics, Department of Quantum Electronics, Laser Research Center, Sauletekio Avenue 10, LT-10223 Vilnius, Lithuania

Evaldas Balciunas, Rasa Jarasiene, Daiva Baltriukiene, Virginija Bukelskiene

Vilnius University, Institute of Biochemistry, Vivarium, Mokslininku Str. 12, LT-08662 Vilnius, Lithuania

Antanas Kraniauskas, Raimondas Sirmenis

Vilnius University, Hospital Santariskiu Klinikos, Heart Surgery Center, Santariskiu Str. 2, LT-08661 Vilnius, Lithuania

J. Biomed. Opt. 17(8), 081405 (May 15, 2012). doi:10.1117/1.JBO.17.8.081405
History: Received December 23, 2011; Revised March 12, 2012; Accepted March 21, 2012
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Abstract.  This work presents the latest results on direct laser writing of polymeric materials for tissue engineering applications. A femtosecond Yb:KGW laser (300 fs, 200 kHz, 515 nm) was used as a light source for non-linear lithography. Fabrication was implemented in various photosensitive polymeric materials, such as: hybrid organic-inorganic sol-gel based on silicon-zirconium oxides, commercial ORMOCER® class photoresins. These materials were structured via multi-photon polymerization technique with submicron resolution. Porous three-dimensional scaffolds for artificial tissue engineering were fabricated with constructed system and were up to several millimeters in overall size with 10 to 100 μm internal pores. Biocompatibility of the used materials was tested in primary rabbit muscle-derived stem cell culture in vitro and using laboratory rats in vivo. This interdisciplinary study suggests that proposed technique and materials are suitable for tissue engineering applications.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Paulius Danilevicius ; Sima Rekstyte ; Evaldas Balciunas ; Antanas Kraniauskas ; Rasa Jarasiene, et al.
"Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering", J. Biomed. Opt. 17(8), 081405 (May 15, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.081405


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