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Research Papers: Imaging

Visualization of the solubilization process of the plasma membrane of a living cell by waveguide evanescent field fluorescence microscopy

[+] Author Affiliations
Abdollah Hassanzadeh, Silvia Mittler

Western University, London, Department of Physics and Astronomy, Ontario, Canada

Heun Kan Ma

Western University, London, Department of Physics and Astronomy, Ontario, Canada

Western University, London, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Ontario, Canada

S. Jeffrey Dixon

Western University, London, Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Ontario, Canada

J. Biomed. Opt. 17(7), 076025 (Jul 24, 2012). doi:10.1117/1.JBO.17.7.076025
History: Received February 9, 2012; Revised May 24, 2012; Accepted June 1, 2012
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Abstract.  Waveguide evanescent field fluorescence microscopy (WEFF) is a novel microscopy technology that allows imaging of a cell’s plasma membrane in the vicinity of a glass substrate with high axial resolution, low background and little photobleaching. Time-lapse imaging can be performed to investigate changes in cell morphology in the presence or absence of chemical agents. WEFF microscopy provides a method to investigate plasma membranes of living cells and allows a comparison to simplified model membranes immobilized on planar substrates. The interaction of the nonionic detergent Triton X-100 with plasma membranes of osteoblasts in an aqueous environment was investigated. Solubilization of the membranes very close to the waveguide surface was visualized and related to the three-stage solubilisation model proposed for liposomes and supported lipid bilayers. Findings for the plasma membranes of cells are in excellent agreement with results reported for these artificial model systems.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Abdollah Hassanzadeh ; Heun Kan Ma ; S. Jeffrey Dixon and Silvia Mittler
"Visualization of the solubilization process of the plasma membrane of a living cell by waveguide evanescent field fluorescence microscopy", J. Biomed. Opt. 17(7), 076025 (Jul 24, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.076025


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