Optical imaging techniques such as multiphoton and confocal microscopy have greatly contributed to our understanding of biological processes in whole organisms. Recent advances in imaging techniques and hardware components have enabled higher resolution imaging at the subcellular level, greater coverage, and superior signal to noise ratios. While such achievements have resulted in unique opportunities to explore biology in living systems, high resolution in vivo imaging continues to be challenged by problems such as insufficient tissue penetration, difficulties in physically accessing organs within a living animal, and tissue movement. Penetration depth and internal access could in part be addressed by using multiphoton microscopy or miniature objectives.1 Tissue movement, however, is unavoidable as it is the result of vital physiological processes such as respiration and cardiovascular activity. Movement arising from respiration is particularly extensive and has effects on each and every organ of the body and every organ of the body such as tibila anterior,2 lung,3 kidney,4 spinal cord,5 and brain.6,7 Because the time taken to acquire an image is typically the same as or longer than a breathing cycle (approx. 400 ms), respiration can have a very detrimental effect on image acquisition, causing severe image distortions and unstable imaging conditions.2 Faster image acquisition would be one way to overcome this problem, but would likely come at the expense of image quality (high signal-to-noise ratios and/or reduced image sizes).