Figure 1(a) shows the mean Raman spectra of normal and malignant tumor tissues in the nasopharynx which were measured under the same instrumentation set-up. Prominent Raman bands are observed in both normal and nasopharyngeal tissues at the following peak positions: [ of proline], [ in -helix conformation of proline and valine], [ breathing of phenylalanine], 1094, 1209, 1268, [ of lipids and proteins], ( wagging mode of proteins and nucleic acids), [ of phospholipids and collagen], [ of lipid], etc. The normalized intensities of Raman peaks at 1094, 1209, 1268, 1290-1340, 1579, and are more intense for NPC tissue than for normal tissue, while Raman bands at 853 and are greater in normal nasopharyngeal samples. Independent-sample -test showed that the differences are significant for the 853, 937, 1094, 1209, 1268, and bands (), while the differences for the 1290 to 1340 and bands are not statistically significant (). Figure 1(b) shows the mean Raman intensity for the two different tissue groups along with corresponding standard deviations at the major Raman bands mentioned above. The six bands (853, 937, 1094, 1209, 1268, and ) with significantly () mean intensity differences between the cancer group and the normal group are labeled with elipitical circles in Fig. 1(b). The band intensities located at 853 and were significantly lower for cancerous tissues (), while the band intensities located at 1094, 1209, 1268, and were significantly higher (). These intensity differences indicate that there is a significant increase and decrease in the percentage of distinctive biomolecules relative to the total Raman-active constituents in different tissue types, suggesting the diagnostic potential of Raman spectroscopy for identification of malignant lesions in the nasopharynx.