Phantoms consisted of a mixture of Intralipid 20% (Fresenius, Kabi AG, Bad Homburg, Germany), phosphate buffered saline, and Evans Blue (Sigma Aldrich, St. Louis, MO) as absorber.5 The refractive index was assumed to be 1.35. The of each phantom was controlled by varying the concentration of Evans Blue, and the was controlled by varying the amount of Intralipid 20%. For high Intralipid concentrations (), was corrected for dependent scattering effects.10 Evans Blue has an absorption of at 611 nm. Intralipid 20% has at 611 nm.11 Phantoms were constructed with , 0.2, 0.4, 1, 2, 3, 5, 10, , at and , and a separate set was constructed with , 0.5, 1, 2, 3.8, 5.5, 8.9, 11.48, 12.75, 13.6, , at and . Additional phantoms were prepared at each without Evans Blue added, and utilized as baseline measurements, . Finally, the reflectance ratio was calculated.