Research Papers: Imaging

Laser line scanning for fluorescence reflectance imaging: a phantom study and in vivo validation of the enhancement of contrast and resolution

[+] Author Affiliations
Frédéric Fantoni, Lionel Hervé, Vincent Poher, Jean-Marc Dinten

Université Grenoble Alpes, 38000 Grenoble, France

CEA, LETI, Minatec Campus, 38054 Grenoble, France

Sylvain Gioux

Harvard Medical School, Beth Israel Deaconess Medical Center, Department of Medicine, Boston, Massachusetts 02215, United States

Jérôme I. Mars

GIPSA Lab, UMR 5216 CNRS—Grenoble INP—Université Joseph Fourier—Université Stendhal, 11 rue des Mathématiques, 38402 Saint Martin D'Heres, France

J. Biomed. Opt. 19(10), 106003 (Oct 01, 2014). doi:10.1117/1.JBO.19.10.106003
History: Received June 26, 2014; Revised August 19, 2014; Accepted September 3, 2014
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Abstract.  Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality to noninvasively monitor fluorescence-targeted tumors. In some situations, this kind of imaging suffers from poor resolution due to the diffusive nature of photons in tissue. The objective of the proposed technique is to tackle this limitation. It relies on the scanning of the medium with a laser line illumination and the acquisition of images at each position of excitation. The detection scheme proposed takes advantage of the stack of images acquired to enhance the resolution and the contrast of the final image. The experimental protocol is described to fully understand why we overpass the classical limits and validate the scheme on tissue-like phantoms and in vivo with a preliminary testing. The results are compared with those obtained with a classical wide-field illumination.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Frédéric Fantoni ; Lionel Hervé ; Vincent Poher ; Sylvain Gioux ; Jérôme I. Mars, et al.
"Laser line scanning for fluorescence reflectance imaging: a phantom study and in vivo validation of the enhancement of contrast and resolution", J. Biomed. Opt. 19(10), 106003 (Oct 01, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.10.106003


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