Special Section on Laser Applications in Life Sciences

Noninvasive monitoring of photodynamic therapy on skin neoplastic lesions using the optical attenuation coefficient measured by optical coherence tomography

[+] Author Affiliations
Viviane P. Goulart

IPEN-CNEN/SP, Center for Lasers and Applications—CLA, Laboratório de Biofotônica, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, SP, Brazil

Moisés O. dos Santos

Universidade do Estado do Amazonas, Escola Superior de Tecnologia, Av. Darcy Vargas, 1200, Parque 10 de Novembro, Manaus, 69050-020, AM, Brazil

Anne Latrive

IPEN-CNEN/SP, Center for Lasers and Applications—CLA, Laboratório de Biofotônica, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, SP, Brazil

Anderson Z. Freitas

IPEN-CNEN/SP, Center for Lasers and Applications—CLA, Laboratório de Biofotônica, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, SP, Brazil

Luciana Correa

Universidade de Sao Paulo, Faculdade de Odontologia, Av. Lineu Prestes, 2227, Sao Paulo, 05508-000, SP, Brazil

Denise M. Zezell

IPEN-CNEN/SP, Center for Lasers and Applications—CLA, Laboratório de Biofotônica, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, SP, Brazil

J. Biomed. Opt. 20(5), 051007 (Nov 21, 2014). doi:10.1117/1.JBO.20.5.051007
History: Received September 1, 2014; Accepted October 24, 2014
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Abstract.  Photodynamic therapy (PDT) has become a promising alternative for treatment of skin lesions such as squamous cell carcinoma. We propose a method to monitor the effects of PDT in a noninvasive way by using the optical attenuation coefficient (OAC) calculated from optical coherence tomography (OCT) images. We conducted a study on mice with chemically induced neoplastic lesions and performed PDT on these lesions using homemade photosensitizers. The response of neoplastic lesions to therapy was monitored using, at the same time, macroscopic clinical visualization, histopathological analysis, OCT imaging, and OCT-based attenuation coefficient measurement. Results with all four modalities demonstrated a positive response to treatment. The attenuation coefficient was found to be 1.4 higher in skin lesions than in healthy tissue and it decreased after therapy. This study shows that the OAC is a potential tool to noninvasively assess the evolution of skin neoplastic lesions with time after treatment.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Viviane P. Goulart ; Moisés O. dos Santos ; Anne Latrive ; Anderson Z. Freitas ; Luciana Correa, et al.
"Noninvasive monitoring of photodynamic therapy on skin neoplastic lesions using the optical attenuation coefficient measured by optical coherence tomography", J. Biomed. Opt. 20(5), 051007 (Nov 21, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051007


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