Research Papers: Imaging

Confocal Raman microscopic investigation of the effectiveness of penetration enhancers for procaine delivery to the skin

[+] Author Affiliations
Dominique Lunter

Eberhard Karls University of Tuebingen, Pharmaceutical Technology, Auf der Morgenstelle 8, 72076 Tuebingen, Germany

Rolf Daniels

Eberhard Karls University of Tuebingen, Pharmaceutical Technology, Auf der Morgenstelle 8, 72076 Tuebingen, Germany

J. Biomed. Opt. 19(12), 126015 (Dec 24, 2014). doi:10.1117/1.JBO.19.12.126015
History: Received June 22, 2014; Accepted November 18, 2014
Text Size: A A A

Abstract.  A methodology that employs confocal Raman microscopy (CRM) on ex vivo skin samples is proposed for the investigation of drug content and distribution in the skin. To this end, the influence of the penetration enhancers propylene glycol and polyoxyethylene-23-lauryl ether on the penetration and permeation of procaine as a model substance was investigated. The drug content of skin samples that had been incubated with semisolid formulations containing one of these enhancers was examined after skin segmentation. The experiments showed that propylene glycol did not affect the procaine content that was delivered to the skin, whereas polyoxyethylene-23-lauryl ether led to higher procaine contents and deeper penetration. Neither substance was found to influence the permeation rate of procaine. It is thereby shown that CRM can provide additional information on drug penetration and permeation. Furthermore, the method was found to enhance the depth from which Raman spectra can be collected and to improve the depth resolution compared to previously proposed methods.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Skin

Citation

Dominique Lunter and Rolf Daniels
"Confocal Raman microscopic investigation of the effectiveness of penetration enhancers for procaine delivery to the skin", J. Biomed. Opt. 19(12), 126015 (Dec 24, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.12.126015


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.