Research Papers: General

Damage induced in red blood cells by infrared optical trapping: an evaluation based on elasticity measurements

[+] Author Affiliations
Marcos A. S. de Oliveira, Adriana Fontes, Renato E. de Araujo

Federal University of Pernambuco, Laboratory of Biomedical Optics and Imaging, Avenida da Arquitetura, s/n, Cidade Universitária, Recife, Pernambuco 50740-530, Brazil

Diógenes S. Moura

Federal University of Pernambuco, Laboratory of Biomedical Optics and Imaging, Avenida da Arquitetura, s/n, Cidade Universitária, Recife, Pernambuco 50740-530, Brazil

Federal University of Pernambuco, Colégio de Aplicação, Avenida da Arquitetura, s/n, Cidade Universitária, Recife, Pernambuco 50740-530, Brazil

J. Biomed. Opt. 21(7), 075012 (Jul 19, 2016). doi:10.1117/1.JBO.21.7.075012
History: Received January 25, 2016; Accepted July 1, 2016
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Abstract.  We evaluated the damage caused to optically trapped red blood cells (RBCs) after 1 or 2 min of exposure to near-infrared (NIR) laser beams at 785 or 1064 nm. Damage was quantified by measuring cell elasticity using an automatic, real-time, homemade, optical tweezer system. The measurements, performed on a significant number (hundreds) of cells, revealed an overall deformability decrease up to 104% after 2 min of light exposure, under 10 mW optical trapping for the 785-nm wavelength. Wavelength dependence of the optical damage is attributed to the light absorption by hemoglobin. The results provided evidence that RBCs have their biomechanical properties affected by NIR radiation. Our findings establish limits for laser applications with RBCs.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Marcos A. S. de Oliveira ; Diógenes S. Moura ; Adriana Fontes and Renato E. de Araujo
"Damage induced in red blood cells by infrared optical trapping: an evaluation based on elasticity measurements", J. Biomed. Opt. 21(7), 075012 (Jul 19, 2016). ; http://dx.doi.org/10.1117/1.JBO.21.7.075012


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